Cloning of DNA oligo pools for in vitro expression

Anna Uzonyi; Ronit Nir; Schraga Schwartz

doi:10.1016/j.xpro.2021.101103

Cloning of DNA oligo pools for in vitro expression

Anna Uzonyi, Ronit Nir, Schraga Schwartz

Department of Molecular Genetics

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Oligo library pools are powerful tools for systematic investigation of genetic and transcriptomic machinery such as promoter function and gene regulation, non-coding RNAs, or RNA modifications. Here, we provide a detailed protocol for cloning DNA oligo pools made up of tens of thousands of different constructs, aiming to preserve the complexity of the pools. This system would be suitable for expression in cell lines and can be followed up by next-generation sequencing analysis.

Original language	English
Article number	101103
Number of pages	17
Journal	STAR Protocols
Volume	3
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2021.101103
State	Published - 18 Mar 2022

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Cloning of DNA oligo pools for in vitro expression

Abstract

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