TY - JOUR
T1 - Classical monocyte ontogeny dictates their functions and fates as tissue macrophages
AU - Trzebanski, Sébastien
AU - Kim, Jung-Seok
AU - Larossi, Niss
AU - Raanan, Ayala
AU - Kancheva, Daliya
AU - Bastos, Jonathan
AU - Haddad, Montaser
AU - Solomon, Aryeh
AU - Sivan, Ehud
AU - Aizik, Dan
AU - Kralova, Jarmila Sekeresova
AU - Gross-Vered, Mor
AU - Boura-Halfon, Sigalit
AU - Lapidot, Tsvee
AU - Alon, Ronen
AU - Movahedi, Kiavash
AU - Jung, Steffen
N1 - We would like to thank all members of the Jung laboratory, as well as the Jung lab alumni A. Mildner and S. Yona, for their advice and comments on the manuscript. We thank F. Ginhoux, Singapore Immunology Network, for sharing the Ms4a3Cre mice and M. Mandelboim, Sheba Hospital, for the provision of and help with the titration of the PR8 virus. We thank the staff of the Weizmann animal and flow cytometry facilities. S.J. is the incumbent of the Henry H. Drake Professional Chair of Immunology. This research was supported by the Morris Kahn Institute for Human Immunology and funded by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation), project-ID 259373024 – TRR 167, the Vienna Science and Technology Fund (WWTF), project 713745, and the Israel Science Foundation (ISF) (grant # 696/21).
PY - 2024/5/14
Y1 - 2024/5/14
N2 - Summary Classical monocytes (CMs) are ephemeral myeloid immune cells that circulate in the blood. Emerging evidence suggests that CMs can have distinct ontogeny and originate from either granulocyte-monocyte- or monocyte-dendritic-cell progenitors (GMPs or MDPs). Here, we report surface markers that allowed segregation of murine GMP- and MDP-derived CMs, i.e., GMP-Mo and MDP-Mo, as well as their functional characterization, including fate definition following adoptive cell transfer. GMP-Mo and MDP-Mo yielded an equal increase in homeostatic CM progeny, such as blood-resident non-classical monocytes and gut macrophages; however, these cells differentially seeded various other selected tissues, including the dura mater and lung. Specifically, GMP-Mo and MDP-Mo differentiated into distinct interstitial lung macrophages, linking CM dichotomy to previously reported pulmonary macrophage heterogeneity. Collectively, we provide evidence for the existence of two functionally distinct CM subsets in the mouse that differentially contribute to peripheral tissue macrophage populations in homeostasis and following challenge.
AB - Summary Classical monocytes (CMs) are ephemeral myeloid immune cells that circulate in the blood. Emerging evidence suggests that CMs can have distinct ontogeny and originate from either granulocyte-monocyte- or monocyte-dendritic-cell progenitors (GMPs or MDPs). Here, we report surface markers that allowed segregation of murine GMP- and MDP-derived CMs, i.e., GMP-Mo and MDP-Mo, as well as their functional characterization, including fate definition following adoptive cell transfer. GMP-Mo and MDP-Mo yielded an equal increase in homeostatic CM progeny, such as blood-resident non-classical monocytes and gut macrophages; however, these cells differentially seeded various other selected tissues, including the dura mater and lung. Specifically, GMP-Mo and MDP-Mo differentiated into distinct interstitial lung macrophages, linking CM dichotomy to previously reported pulmonary macrophage heterogeneity. Collectively, we provide evidence for the existence of two functionally distinct CM subsets in the mouse that differentially contribute to peripheral tissue macrophage populations in homeostasis and following challenge.
UR - http://www.scopus.com/inward/record.url?scp=85194560568&partnerID=8YFLogxK
U2 - https://doi.org/10.1016/j.immuni.2024.04.019
DO - https://doi.org/10.1016/j.immuni.2024.04.019
M3 - مقالة
C2 - 38749446
SN - 1074-7613
VL - 57
SP - 1225-1242.e6
JO - Immunity
JF - Immunity
IS - 6
ER -