Characterizing Rare Dormant and Cycling Lineages Using the Watermelon System

Abram Handly-Santana, Yaara Oren

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Barcode-based lineage tracing approaches enable molecular characterization of clonal cell families. Barcodes that are expressed as mRNA can be used to deconvolve lineage identity from single-cell RNA sequencing transcriptional data. Here we describe the Watermelon system, which facilitates the simultaneous tracing of lineage, transcriptional, and proliferative state at a single cell level.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages165-175
Number of pages11
DOIs
StatePublished - 2024

Publication series

NameMethods in Molecular Biology
Volume2811

Keywords

  • Expressed barcodes
  • Lineage tracing
  • Proliferation tracking
  • Single-cell RNA sequencing (scRNA-seq)

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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