Characterization of tilapia (Oreochromis niloticus) gonadotropins by modeling and immunoneutralization

Joseph Aizen, Noga Kowalsman, Masha Y. Niv, Berta Levavi-Sivan

Research output: Contribution to journalArticlepeer-review

Abstract

In fish, both follicle-stimulating hormone (FSH) and luteinizing hormone (LH) play important roles in reproduction. Here we explored the structure and differential specificity of tilapia (t) gonadotropins (GTHs) to delineate their physiological relevance and the nature of their regulation. We generated structural models of tGTHs and GTH receptors (R) that enabled us to better understand the hormone-receptor interacting region. In tilapia, FSH release is under the control of the hypothalamic decapeptide GnRH, an effect that was abolished by specific bioneutralizing antisera [anti-recombinant (r) tFSHβ]. These antisera also reduced the basal secretion and delayed GnRH-stimulated production of 11-ketotestosterone (11KT), and dramatically reduced LH levels. Immunoneutralization of tLH using anti-rtLHβ significantly reduced its GnRH-stimulated levels. Basal 11KT and FSH levels were also reduced. Taken together, these results suggest a feedback mechanism between FSH and LH release in tilapia.

Original languageAmerican English
Pages (from-to)28-33
Number of pages6
JournalGeneral and Comparative Endocrinology
Volume207
DOIs
StatePublished - 1 Oct 2014

Keywords

  • 11-ketotestosterone
  • FSH
  • GnRH
  • Gonadotropin receptor
  • LH

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology
  • Endocrinology

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