Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato

Evyatar Steiner; Alon Israeli; Rupali Gupta; Ido Shwartz; Ido Nir; Meirav Leibman-Markus; Lior Tal; Mika Farber; Ziva Amsalem; Naomi Ori; Bruno Müller; Maya Bar

doi:https://doi.org/10.1186/s13007-020-00694-2

Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato

Evyatar Steiner, Alon Israeli, Rupali Gupta, Ido Shwartz, Ido Nir, Meirav Leibman-Markus, Lior Tal, Mika Farber, Ziva Amsalem, Naomi Ori, Bruno Müller, Maya Bar

Weizmann Institute of Science, The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Background: Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development. Results: Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato. Conclusions: The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers.

Original language	American English
Article number	152
Number of pages	12
Journal	Plant Methods
Volume	16
Issue number	1
Early online date	16 Nov 2020
DOIs	https://doi.org/10.1186/s13007-020-00694-2
State	Published - 1 Dec 2020

Keywords

Arabidopsis
Cytokinin
Imaging
TCSv2
Tomato
Two-component sensor (TCS)

All Science Journal Classification (ASJC) codes

Biotechnology
Genetics
Plant Science

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https://doi.org/10.1186/s13007-020-00694-2

Cite this

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title = "Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato",

abstract = "Background: Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development. Results: Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato. Conclusions: The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers.",

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AU - Steiner, Evyatar

AU - Israeli, Alon

AU - Gupta, Rupali

AU - Shwartz, Ido

AU - Nir, Ido

AU - Leibman-Markus, Meirav

AU - Tal, Lior

AU - Farber, Mika

AU - Amsalem, Ziva

AU - Ori, Naomi

AU - Müller, Bruno

AU - Bar, Maya

PY - 2020/12/1

Y1 - 2020/12/1

N2 - Background: Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development. Results: Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato. Conclusions: The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers.

AB - Background: Hormones are crucial to plant life and development. Being able to follow the plants hormonal response to various stimuli and throughout developmental processes is an important and increasingly widespread tool. The phytohormone cytokinin (CK) has crucial roles in the regulation of plant growth and development. Results: Here we describe a version of the CK sensor Two Component signaling Sensor (TCS), referred to as TCSv2. TCSv2 has a different arrangement of binding motifs when compared to previous TCS versions, resulting in increased sensitivity in some examined tissues. Here, we examine the CK responsiveness and distribution pattern of TCSv2 in arabidopsis and tomato. Conclusions: The increased sensitivity and reported expression pattern of TCSv2 make it an ideal TCS version to study CK response in particular hosts, such as tomato, and particular tissues, such as leaves and flowers.

KW - Arabidopsis

KW - Cytokinin

KW - Imaging

KW - TCSv2

KW - Tomato

KW - Two-component sensor (TCS)

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Characterization of the cytokinin sensor TCSv2 in arabidopsis and tomato

Abstract

Keywords

All Science Journal Classification (ASJC) codes

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