Characterization of a novel orthomyxo-like virus causing mass die-offs of Tilapia

Eran Bacharach; Nischay Mishra; Thomas Briese; Michael C. Zody; Japhette Esther Kembou Tsofack; Rachel Zamostiano; Asaf Berkowitz; James Ng; Adam Nitido; André Corvelo; Nora C. Toussaint; Sandra Cathrine Abel Nielsen; Mady Hornig; Jorge del Pozo; Toby Bloom; Hugh Ferguson; Avi Eldar; W. Ian Lipkin

doi:https://doi.org/10.1128/mBio.00431-16

Characterization of a novel orthomyxo-like virus causing mass die-offs of Tilapia

Eran Bacharach, Nischay Mishra, Thomas Briese, Michael C. Zody, Japhette Esther Kembou Tsofack, Rachel Zamostiano, Asaf Berkowitz, James Ng, Adam Nitido, André Corvelo, Nora C. Toussaint, Sandra Cathrine Abel Nielsen, Mady Hornig, Jorge del Pozo, Toby Bloom, Hugh Ferguson, Avi Eldar, W. Ian Lipkin

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

Tilapia are an important global food source due to their omnivorous diet, tolerance for high-density aquaculture, and relative disease resistance. Since 2009, tilapia aquaculture has been threatened by mass die-offs in farmed fish in Israel and Ecuador. Here we report evidence implicating a novel orthomyxo-like virus in these outbreaks. The tilapia lake virus (TiLV) has a 10-segment, negative-sense RNA genome. The largest segment, segment 1, contains an open reading frame with weak sequence homology to the influenza C virus PB1 subunit. The other nine segments showed no homology to other viruses but have conserved, complementary sequences at their 5’ and 3’ termini, consistent with the genome organization found in other orthomyxoviruses. In situ hybridization indicates TiLV replication and transcription at sites of pathology in the liver and central nervous system of tilapia with disease.

Original language	English
Article number	e00431-16
Journal	mBio
Volume	7
Issue number	2
DOIs	https://doi.org/10.1128/mBio.00431-16
State	Published - 5 Apr 2016

All Science Journal Classification (ASJC) codes

Virology
Microbiology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.1128/mBio.00431-16

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Bacharach, E., Mishra, N., Briese, T., Zody, M. C., Tsofack, J. E. K., Zamostiano, R., Berkowitz, A., Ng, J., Nitido, A., Corvelo, A., Toussaint, N. C., Abel Nielsen, S. C., Hornig, M., del Pozo, J., Bloom, T., Ferguson, H., Eldar, A., & Lipkin, W. I. (2016). Characterization of a novel orthomyxo-like virus causing mass die-offs of Tilapia. mBio, 7(2), Article e00431-16. https://doi.org/10.1128/mBio.00431-16

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title = "Characterization of a novel orthomyxo-like virus causing mass die-offs of Tilapia",

abstract = "Tilapia are an important global food source due to their omnivorous diet, tolerance for high-density aquaculture, and relative disease resistance. Since 2009, tilapia aquaculture has been threatened by mass die-offs in farmed fish in Israel and Ecuador. Here we report evidence implicating a novel orthomyxo-like virus in these outbreaks. The tilapia lake virus (TiLV) has a 10-segment, negative-sense RNA genome. The largest segment, segment 1, contains an open reading frame with weak sequence homology to the influenza C virus PB1 subunit. The other nine segments showed no homology to other viruses but have conserved, complementary sequences at their 5{\textquoteright} and 3{\textquoteright} termini, consistent with the genome organization found in other orthomyxoviruses. In situ hybridization indicates TiLV replication and transcription at sites of pathology in the liver and central nervous system of tilapia with disease.",

author = "Eran Bacharach and Nischay Mishra and Thomas Briese and Zody, {Michael C.} and Tsofack, {Japhette Esther Kembou} and Rachel Zamostiano and Asaf Berkowitz and James Ng and Adam Nitido and Andr{\'e} Corvelo and Toussaint, {Nora C.} and {Abel Nielsen}, {Sandra Cathrine} and Mady Hornig and {del Pozo}, Jorge and Toby Bloom and Hugh Ferguson and Avi Eldar and Lipkin, {W. Ian}",

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year = "2016",

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doi = "https://doi.org/10.1128/mBio.00431-16",

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Bacharach, E, Mishra, N, Briese, T, Zody, MC, Tsofack, JEK, Zamostiano, R, Berkowitz, A, Ng, J, Nitido, A, Corvelo, A, Toussaint, NC, Abel Nielsen, SC, Hornig, M, del Pozo, J, Bloom, T, Ferguson, H, Eldar, A & Lipkin, WI 2016, 'Characterization of a novel orthomyxo-like virus causing mass die-offs of Tilapia', mBio, vol. 7, no. 2, e00431-16. https://doi.org/10.1128/mBio.00431-16

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T1 - Characterization of a novel orthomyxo-like virus causing mass die-offs of Tilapia

AU - Bacharach, Eran

AU - Mishra, Nischay

AU - Briese, Thomas

AU - Zody, Michael C.

AU - Tsofack, Japhette Esther Kembou

AU - Zamostiano, Rachel

AU - Berkowitz, Asaf

AU - Ng, James

AU - Nitido, Adam

AU - Corvelo, André

AU - Toussaint, Nora C.

AU - Abel Nielsen, Sandra Cathrine

AU - Hornig, Mady

AU - del Pozo, Jorge

AU - Bloom, Toby

AU - Ferguson, Hugh

AU - Eldar, Avi

AU - Lipkin, W. Ian

PY - 2016/4/5

Y1 - 2016/4/5

N2 - Tilapia are an important global food source due to their omnivorous diet, tolerance for high-density aquaculture, and relative disease resistance. Since 2009, tilapia aquaculture has been threatened by mass die-offs in farmed fish in Israel and Ecuador. Here we report evidence implicating a novel orthomyxo-like virus in these outbreaks. The tilapia lake virus (TiLV) has a 10-segment, negative-sense RNA genome. The largest segment, segment 1, contains an open reading frame with weak sequence homology to the influenza C virus PB1 subunit. The other nine segments showed no homology to other viruses but have conserved, complementary sequences at their 5’ and 3’ termini, consistent with the genome organization found in other orthomyxoviruses. In situ hybridization indicates TiLV replication and transcription at sites of pathology in the liver and central nervous system of tilapia with disease.

AB - Tilapia are an important global food source due to their omnivorous diet, tolerance for high-density aquaculture, and relative disease resistance. Since 2009, tilapia aquaculture has been threatened by mass die-offs in farmed fish in Israel and Ecuador. Here we report evidence implicating a novel orthomyxo-like virus in these outbreaks. The tilapia lake virus (TiLV) has a 10-segment, negative-sense RNA genome. The largest segment, segment 1, contains an open reading frame with weak sequence homology to the influenza C virus PB1 subunit. The other nine segments showed no homology to other viruses but have conserved, complementary sequences at their 5’ and 3’ termini, consistent with the genome organization found in other orthomyxoviruses. In situ hybridization indicates TiLV replication and transcription at sites of pathology in the liver and central nervous system of tilapia with disease.

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ER -

Characterization of a novel orthomyxo-like virus causing mass die-offs of Tilapia

Abstract

All Science Journal Classification (ASJC) codes

UN SDGs

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