Cellular internalization pathways of glucose-functionalized gold nanoparticles

Chen Tzror-Azankot; Tamar Dreifuss; Tal Shachar Ben-Gal; Avi Jacob; Tamar Sadan; Menachem Motiei; Rachela Popovtzer

doi:10.1117/12.2509605

Cellular internalization pathways of glucose-functionalized gold nanoparticles

Chen Tzror-Azankot, Tamar Dreifuss, Tal Shachar Ben-Gal, Avi Jacob, Tamar Sadan, Menachem Motiei, Rachela Popovtzer

Bar-Ilan University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Nanomaterials functionalized with glucose have shown a great potential in cancer diagnosis and treatment. We have recently demonstrated that gold nanoparticles (GNP), functionalized with glucose, can be used for specific and sensitive tumor detection when combined with computed tomography (CT)-GNP functionalized with glucose in the second carbon position were used as a metabolically targeted contrast agents, and were able to discriminate between cancer and inflammation, which is a superior ability when comparing the FDG-PET which is not tumor specific. Here we aim to understand the uptake mechanisms of the glucose functionalized GNP using a comprehensive in vitro study in several cell types with different metabolic features, and examining the glucose transporter-1 (GLUT1) involvement in the uptake process. We found that the glucose functionalized GNP are not toxic to the cells in the tested concentrations and we demonstrate that the cellular uptake of GNP, when functionalized with glucose in the second carbon position, strongly depends on GLUT1 expression in the cells, which triggers clathrin-mediated endocytosis of the nanoparticles. This study can promote control and development of glucose-functionalized nanoparticles for many biological applications.

Original language	English
Title of host publication	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI
Editors	Dan V. Nicolau, Dror Fixler, Ewa M. Goldys
Publisher	SPIE
ISBN (Electronic)	9781510624245
DOIs	https://doi.org/10.1117/12.2509605
State	Published - 2019
Event	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019 - San Francisco, United States Duration: 3 Feb 2019 → 4 Feb 2019

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	10891

Conference

Conference	Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019
Country/Territory	United States
City	San Francisco
Period	3/02/19 → 4/02/19

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Cancer
Clathrinmediated endocytosis
GLUT-1
Glucose-coated nanoparticles
Gold nanoparticles
Metabolic-targeting

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2509605

Cite this

Tzror-Azankot, C., Dreifuss, T., Ben-Gal, T. S., Jacob, A., Sadan, T., Motiei, M., & Popovtzer, R. (2019). Cellular internalization pathways of glucose-functionalized gold nanoparticles. In D. V. Nicolau, D. Fixler, & E. M. Goldys (Eds.), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI Article 108911E (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10891). SPIE. https://doi.org/10.1117/12.2509605

@inproceedings{e0bbba2c5e394f298c5d2acf636914a6,

title = "Cellular internalization pathways of glucose-functionalized gold nanoparticles",

abstract = "Nanomaterials functionalized with glucose have shown a great potential in cancer diagnosis and treatment. We have recently demonstrated that gold nanoparticles (GNP), functionalized with glucose, can be used for specific and sensitive tumor detection when combined with computed tomography (CT)-GNP functionalized with glucose in the second carbon position were used as a metabolically targeted contrast agents, and were able to discriminate between cancer and inflammation, which is a superior ability when comparing the FDG-PET which is not tumor specific. Here we aim to understand the uptake mechanisms of the glucose functionalized GNP using a comprehensive in vitro study in several cell types with different metabolic features, and examining the glucose transporter-1 (GLUT1) involvement in the uptake process. We found that the glucose functionalized GNP are not toxic to the cells in the tested concentrations and we demonstrate that the cellular uptake of GNP, when functionalized with glucose in the second carbon position, strongly depends on GLUT1 expression in the cells, which triggers clathrin-mediated endocytosis of the nanoparticles. This study can promote control and development of glucose-functionalized nanoparticles for many biological applications.",

keywords = "Cancer, Clathrinmediated endocytosis, GLUT-1, Glucose-coated nanoparticles, Gold nanoparticles, Metabolic-targeting",

author = "Chen Tzror-Azankot and Tamar Dreifuss and Ben-Gal, \{Tal Shachar\} and Avi Jacob and Tamar Sadan and Menachem Motiei and Rachela Popovtzer",

note = "Publisher Copyright: {\textcopyright} 2019 SPIE.; Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019 ; Conference date: 03-02-2019 Through 04-02-2019",

year = "2019",

doi = "10.1117/12.2509605",

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booktitle = "Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI",

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Tzror-Azankot, C, Dreifuss, T, Ben-Gal, TS, Jacob, A, Sadan, T, Motiei, M & Popovtzer, R 2019, Cellular internalization pathways of glucose-functionalized gold nanoparticles. in DV Nicolau, D Fixler & EM Goldys (eds), Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI., 108911E, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 10891, SPIE, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019, San Francisco, United States, 3/02/19. https://doi.org/10.1117/12.2509605

Cellular internalization pathways of glucose-functionalized gold nanoparticles. / Tzror-Azankot, Chen; Dreifuss, Tamar; Ben-Gal, Tal Shachar et al.
Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI. ed. / Dan V. Nicolau; Dror Fixler; Ewa M. Goldys. SPIE, 2019. 108911E (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 10891).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

TY - GEN

T1 - Cellular internalization pathways of glucose-functionalized gold nanoparticles

AU - Tzror-Azankot, Chen

AU - Dreifuss, Tamar

AU - Ben-Gal, Tal Shachar

AU - Jacob, Avi

AU - Sadan, Tamar

AU - Motiei, Menachem

AU - Popovtzer, Rachela

PY - 2019

Y1 - 2019

N2 - Nanomaterials functionalized with glucose have shown a great potential in cancer diagnosis and treatment. We have recently demonstrated that gold nanoparticles (GNP), functionalized with glucose, can be used for specific and sensitive tumor detection when combined with computed tomography (CT)-GNP functionalized with glucose in the second carbon position were used as a metabolically targeted contrast agents, and were able to discriminate between cancer and inflammation, which is a superior ability when comparing the FDG-PET which is not tumor specific. Here we aim to understand the uptake mechanisms of the glucose functionalized GNP using a comprehensive in vitro study in several cell types with different metabolic features, and examining the glucose transporter-1 (GLUT1) involvement in the uptake process. We found that the glucose functionalized GNP are not toxic to the cells in the tested concentrations and we demonstrate that the cellular uptake of GNP, when functionalized with glucose in the second carbon position, strongly depends on GLUT1 expression in the cells, which triggers clathrin-mediated endocytosis of the nanoparticles. This study can promote control and development of glucose-functionalized nanoparticles for many biological applications.

AB - Nanomaterials functionalized with glucose have shown a great potential in cancer diagnosis and treatment. We have recently demonstrated that gold nanoparticles (GNP), functionalized with glucose, can be used for specific and sensitive tumor detection when combined with computed tomography (CT)-GNP functionalized with glucose in the second carbon position were used as a metabolically targeted contrast agents, and were able to discriminate between cancer and inflammation, which is a superior ability when comparing the FDG-PET which is not tumor specific. Here we aim to understand the uptake mechanisms of the glucose functionalized GNP using a comprehensive in vitro study in several cell types with different metabolic features, and examining the glucose transporter-1 (GLUT1) involvement in the uptake process. We found that the glucose functionalized GNP are not toxic to the cells in the tested concentrations and we demonstrate that the cellular uptake of GNP, when functionalized with glucose in the second carbon position, strongly depends on GLUT1 expression in the cells, which triggers clathrin-mediated endocytosis of the nanoparticles. This study can promote control and development of glucose-functionalized nanoparticles for many biological applications.

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KW - Clathrinmediated endocytosis

KW - GLUT-1

KW - Glucose-coated nanoparticles

KW - Gold nanoparticles

KW - Metabolic-targeting

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DO - 10.1117/12.2509605

M3 - منشور من مؤتمر

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI

A2 - Nicolau, Dan V.

A2 - Fixler, Dror

A2 - Goldys, Ewa M.

PB - SPIE

T2 - Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI 2019

Y2 - 3 February 2019 through 4 February 2019

ER -

Tzror-Azankot C, Dreifuss T, Ben-Gal TS, Jacob A, Sadan T, Motiei M et al. Cellular internalization pathways of glucose-functionalized gold nanoparticles. In Nicolau DV, Fixler D, Goldys EM, editors, Nanoscale Imaging, Sensing, and Actuation for Biomedical Applications XVI. SPIE. 2019. 108911E. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2509605

Cellular internalization pathways of glucose-functionalized gold nanoparticles

Abstract

Publication series

Conference

UN SDGs

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

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