TY - JOUR
T1 - Cell-mediated cholesterol crystal processing and clearance observed by 3D cryo-imaging in human atherosclerotic plaques
AU - Capua-Shenkar, Jenny
AU - Kaestner, Antonia
AU - Rechav, Katya
AU - Brumfeld, Vlad
AU - Kaplan-Ashiri, Ifat
AU - Avinoam, Ori
AU - Speter, Chen
AU - Halak, Moshe
AU - Kruth, Howard
AU - Addadi, Lia
N1 - We thank Dr. Neta Varsano for her help with cryo-SEM and image processing. We thank Leslie Leiserowitz and Benjamin Geiger for critical reading of the manuscript. We thank Prof. Irit Sagi for the gift of J774A.1 cells.
PY - 2023/11/29
Y1 - 2023/11/29
N2 - Atherosclerosis is a pathology affecting the arteries, characterized by the buildup of plaques in the blood vessel walls. Atherosclerosis is the main cause of cardiovascular diseases, which constitute the leading cause of death in the world. Cholesterol crystals are the main components of the plaques, which actively participate in plaque growth and rupture and do not dissolve in aqueous environments. Employing novel cryo-scanning electron microscopy techniques, we examined human atherosclerotic plaques at high resolution, in 3D, and in close to native conditions. We show that cholesterol crystal clearance occurs in advanced human plaques through the activity of cells. We suggest that this occurs by enzymatic esterification of cholesterol to cholesteryl ester, which aggregates into intra- and extra-cellular pools. This discovery provides further understanding of the disease process in atherosclerosis, and may inspire new therapeutic approaches.
AB - Atherosclerosis is a pathology affecting the arteries, characterized by the buildup of plaques in the blood vessel walls. Atherosclerosis is the main cause of cardiovascular diseases, which constitute the leading cause of death in the world. Cholesterol crystals are the main components of the plaques, which actively participate in plaque growth and rupture and do not dissolve in aqueous environments. Employing novel cryo-scanning electron microscopy techniques, we examined human atherosclerotic plaques at high resolution, in 3D, and in close to native conditions. We show that cholesterol crystal clearance occurs in advanced human plaques through the activity of cells. We suggest that this occurs by enzymatic esterification of cholesterol to cholesteryl ester, which aggregates into intra- and extra-cellular pools. This discovery provides further understanding of the disease process in atherosclerosis, and may inspire new therapeutic approaches.
U2 - https://doi.org/10.1101/2023.11.28.568890
DO - https://doi.org/10.1101/2023.11.28.568890
M3 - مقالة
SN - 2692-8205
JO - bioRxiv
JF - bioRxiv
ER -