CEACAM1 Activation by CbpF-Expressing E. coli

Amjad Shhadeh; Johanna Galaski; Tamar Alon-Maimon; Jamal Fahoum; Reuven Wiener; Daniel J. Slade; Ofer Mandelboim; Gilad Bachrach

doi:https://doi.org/10.3389/fcimb.2021.699015

CEACAM1 Activation by CbpF-Expressing E. coli

Amjad Shhadeh, Johanna Galaski, Tamar Alon-Maimon, Jamal Fahoum, Reuven Wiener, Daniel J. Slade, Ofer Mandelboim, Gilad Bachrach

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Recent studies on the oral, anaerobic, gram-negative bacterium Fusobacterium nucleatum revealed its presence and involvement in colorectal, esophageal and breast cancer. We previously demonstrated that F. nucleatum binds and activates the human inhibitory receptors TIGIT and CEACAM1 leading to inhibition of T and NK cell anti-tumor immunity. CEACAM1 was found to be bound and activated by the fusobacterial trimeric autotransporter adhesin CbpF. Here we report the generation of a recombinant E. coli expressing full-length CbpF that efficiently binds and activates CEACAM1.

Original language	English
Article number	699015
Journal	Frontiers in Cellular and Infection Microbiology
Volume	11
DOIs	https://doi.org/10.3389/fcimb.2021.699015
State	Published - 29 Jul 2021

Keywords

CEACAM1
CbpF
F. nucleatum
NK cells
trimeric autotransporter adhesin

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.3389/fcimb.2021.699015

Cite this

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title = "CEACAM1 Activation by CbpF-Expressing E. coli",

abstract = "Recent studies on the oral, anaerobic, gram-negative bacterium Fusobacterium nucleatum revealed its presence and involvement in colorectal, esophageal and breast cancer. We previously demonstrated that F. nucleatum binds and activates the human inhibitory receptors TIGIT and CEACAM1 leading to inhibition of T and NK cell anti-tumor immunity. CEACAM1 was found to be bound and activated by the fusobacterial trimeric autotransporter adhesin CbpF. Here we report the generation of a recombinant E. coli expressing full-length CbpF that efficiently binds and activates CEACAM1.",

keywords = "CEACAM1, CbpF, F. nucleatum, NK cells, trimeric autotransporter adhesin",

author = "Amjad Shhadeh and Johanna Galaski and Tamar Alon-Maimon and Jamal Fahoum and Reuven Wiener and Slade, {Daniel J.} and Ofer Mandelboim and Gilad Bachrach",

note = "Publisher Copyright: {\textcopyright} Copyright {\textcopyright} 2021 Shhadeh, Galaski, Alon-Maimon, Fahoum, Wiener, Slade, Mandelboim and Bachrach.",

year = "2021",

month = jul,

day = "29",

doi = "https://doi.org/10.3389/fcimb.2021.699015",

language = "الإنجليزيّة",

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journal = "Frontiers in Cellular and Infection Microbiology",

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TY - JOUR

T1 - CEACAM1 Activation by CbpF-Expressing E. coli

AU - Shhadeh, Amjad

AU - Galaski, Johanna

AU - Alon-Maimon, Tamar

AU - Fahoum, Jamal

AU - Wiener, Reuven

AU - Slade, Daniel J.

AU - Mandelboim, Ofer

AU - Bachrach, Gilad

PY - 2021/7/29

Y1 - 2021/7/29

N2 - Recent studies on the oral, anaerobic, gram-negative bacterium Fusobacterium nucleatum revealed its presence and involvement in colorectal, esophageal and breast cancer. We previously demonstrated that F. nucleatum binds and activates the human inhibitory receptors TIGIT and CEACAM1 leading to inhibition of T and NK cell anti-tumor immunity. CEACAM1 was found to be bound and activated by the fusobacterial trimeric autotransporter adhesin CbpF. Here we report the generation of a recombinant E. coli expressing full-length CbpF that efficiently binds and activates CEACAM1.

AB - Recent studies on the oral, anaerobic, gram-negative bacterium Fusobacterium nucleatum revealed its presence and involvement in colorectal, esophageal and breast cancer. We previously demonstrated that F. nucleatum binds and activates the human inhibitory receptors TIGIT and CEACAM1 leading to inhibition of T and NK cell anti-tumor immunity. CEACAM1 was found to be bound and activated by the fusobacterial trimeric autotransporter adhesin CbpF. Here we report the generation of a recombinant E. coli expressing full-length CbpF that efficiently binds and activates CEACAM1.

KW - CEACAM1

KW - CbpF

KW - F. nucleatum

KW - NK cells

KW - trimeric autotransporter adhesin

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U2 - https://doi.org/10.3389/fcimb.2021.699015

DO - https://doi.org/10.3389/fcimb.2021.699015

M3 - مقالة

C2 - 34395310

SN - 2235-2988

VL - 11

JO - Frontiers in Cellular and Infection Microbiology

JF - Frontiers in Cellular and Infection Microbiology

M1 - 699015

ER -

CEACAM1 Activation by CbpF-Expressing E. coli

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

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