Calcium imaging using transient fluorescence-lifetime imaging by line-scanning TCSPC

Samuel Frere; Inna Slutsky

doi:https://doi.org/10.1007/978-3-319-14929-5_5

Calcium imaging using transient fluorescence-lifetime imaging by line-scanning TCSPC

Samuel Frere, Inna Slutsky

Physiology Pharmacology

Tel Aviv University

Research output: Contribution to journal › Article › peer-review

Abstract

We present a technique that records transient changes in the concentration of free Ca2+ in live neurons with spatial resolution along a one-dimensional scan. The technique is based on recording fluorescence lifetime changes of a Ca2+ probe by multi-dimensional TCSPC. The sample is scanned with a high-frequency pulsed laser beam, single photons of the fluorescence light are detected, and a photon distribution over the distance along the scan, the arrival times of the photons after the excitation pulses and the time after a periodical stimulation of the sample is built up. The maximum resolution at which lifetime changes can be recorded is given by the line scan period. Transient lifetime effects can thus be resolved at a resolution of about 1 ms.

Original language	English
Pages (from-to)	213-224
Number of pages	12
Journal	Springer Series in Chemical Physics
Volume	111
DOIs	https://doi.org/10.1007/978-3-319-14929-5_5
State	Published - 2015

All Science Journal Classification (ASJC) codes

Physical and Theoretical Chemistry

Access to Document

https://doi.org/10.1007/978-3-319-14929-5_5

Cite this

@article{da770b1d98cc43b3b05bfa83f8a63f58,

title = "Calcium imaging using transient fluorescence-lifetime imaging by line-scanning TCSPC",

abstract = "We present a technique that records transient changes in the concentration of free Ca2+ in live neurons with spatial resolution along a one-dimensional scan. The technique is based on recording fluorescence lifetime changes of a Ca2+ probe by multi-dimensional TCSPC. The sample is scanned with a high-frequency pulsed laser beam, single photons of the fluorescence light are detected, and a photon distribution over the distance along the scan, the arrival times of the photons after the excitation pulses and the time after a periodical stimulation of the sample is built up. The maximum resolution at which lifetime changes can be recorded is given by the line scan period. Transient lifetime effects can thus be resolved at a resolution of about 1 ms.",

author = "Samuel Frere and Inna Slutsky",

note = "Publisher Copyright: {\textcopyright} Springer International Publishing Switzerland 2015.",

year = "2015",

doi = "https://doi.org/10.1007/978-3-319-14929-5_5",

language = "الإنجليزيّة",

volume = "111",

pages = "213--224",

journal = "Springer Series in Chemical Physics",

issn = "0172-6218",

publisher = "Springer New York",

}

TY - JOUR

T1 - Calcium imaging using transient fluorescence-lifetime imaging by line-scanning TCSPC

AU - Frere, Samuel

AU - Slutsky, Inna

PY - 2015

Y1 - 2015

N2 - We present a technique that records transient changes in the concentration of free Ca2+ in live neurons with spatial resolution along a one-dimensional scan. The technique is based on recording fluorescence lifetime changes of a Ca2+ probe by multi-dimensional TCSPC. The sample is scanned with a high-frequency pulsed laser beam, single photons of the fluorescence light are detected, and a photon distribution over the distance along the scan, the arrival times of the photons after the excitation pulses and the time after a periodical stimulation of the sample is built up. The maximum resolution at which lifetime changes can be recorded is given by the line scan period. Transient lifetime effects can thus be resolved at a resolution of about 1 ms.

AB - We present a technique that records transient changes in the concentration of free Ca2+ in live neurons with spatial resolution along a one-dimensional scan. The technique is based on recording fluorescence lifetime changes of a Ca2+ probe by multi-dimensional TCSPC. The sample is scanned with a high-frequency pulsed laser beam, single photons of the fluorescence light are detected, and a photon distribution over the distance along the scan, the arrival times of the photons after the excitation pulses and the time after a periodical stimulation of the sample is built up. The maximum resolution at which lifetime changes can be recorded is given by the line scan period. Transient lifetime effects can thus be resolved at a resolution of about 1 ms.

UR - http://www.scopus.com/inward/record.url?scp=84927932172&partnerID=8YFLogxK

U2 - https://doi.org/10.1007/978-3-319-14929-5_5

DO - https://doi.org/10.1007/978-3-319-14929-5_5

M3 - مقالة

SN - 0172-6218

VL - 111

SP - 213

EP - 224

JO - Springer Series in Chemical Physics

JF - Springer Series in Chemical Physics

ER -

Calcium imaging using transient fluorescence-lifetime imaging by line-scanning TCSPC

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this