@article{0499cc6b150e48aba703a94270ef154e,
title = "Bright and photostable chemigenetic indicators for extended in vivo voltage imaging",
abstract = "Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, the utility of existing GEVIs has been limited by the brightness and photostability of fluorescent proteins and rhodopsins. We engineered a GEVI, called Voltron, that uses bright and photostable synthetic dyes instead of protein-based fluorophores, thereby extending the number of neurons imaged simultaneously in vivo by a factor of 10 and enabling imaging for significantly longer durations relative to existing GEVIs. We used Voltron for in vivo voltage imaging in mice, zebrafish, and fruit flies. In the mouse cortex, Voltron allowed single-trial recording of spikes and subthreshold voltage signals from dozens of neurons simultaneously over a 15-minute period of continuous imaging. In larval zebrafish, Voltron enabled the precise correlation of spike timing with behavior.",
author = "Abdelfattah, {Ahmed S.} and Takashi Kawashima and Amrita Singh and Ondrej Novak and Hui Liu and Yichun Shuai and Yi-Chieh Huang and Luke Campagnola and Seeman, {Stephanie C.} and Jianing Yu and Jihong Zheng and Grimm, {Jonathan B.} and Ronak Patel and Johannes Friedrich and Mensh, {Brett D.} and Liam Paninski and Macklin, {John J.} and Murphy, {Gabe J.} and Kaspar Podgorski and Bei-Jung Lin and Tsai-Wen Chen and Turner, {Glenn C.} and Zhe Liu and Minoru Koyama and Karel Svoboda and Ahrens, {Misha B.} and Lavis, {Luke D.} and Schreiter, {Eric R.}",
note = "We thank the Vivarium, Cell Culture, Instrument Design and Fabrication, Imaging, Molecular Biology, and Virus Production facilities at Janelia for assistance. Specifically, we thank B. Shields, D. Walpita, J. Cox, C. McGlynn, D. Alcor, A. Taylor, J. Rouchard, K. Ritola, X. Zhang, and J. Towne. We thank Z. Wei for discussions on data analysis. Funding: Supported by HHMI (A.S.A., T.K., A.S., O.N., H.L., Y.S., J.Y., J.Z., J.B.G., R.P., B.D.M., J.J.M., K.P., G.C.T., Z.L., M.K., K.S., M.B.A., L.D.L., and E.R.S.), Simons Collaboration on the Global Brain research awards 325171 and 542943SPI (M.B.A. and L.P.), IARPA MICRONS D16PC00003 (L.P.), NIH R01EB22913 (L.P.), Taiwan Ministry of Science and Technology MOST106-2628-B-010-004, MOST105-2628-B-010-005, MOST106-2320-B-010-012 and Taiwan National Health Research Institute NHRI-ex-107-10509NC (T.-W.C. and B.-J.L.), and the Allen Institute for Brain Science (L.C., S.C.S., and G.J.M.).",
year = "2019",
month = aug,
day = "16",
doi = "https://doi.org/10.1126/science.aav6416",
language = "الإنجليزيّة",
volume = "365",
pages = "699--704",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6454",
}