@article{c383807925f44c0abd699c38d42304f6,
title = "Brief report: miR-290-295 regulate embryonic stem cell differentiation propensities by repressing pax6",
abstract = "microRNAs of the miR-290-295 family are selectively expressed at high levels in mouse embryonic stem cells (mESCs) and have established roles in regulating self-renewal. However, the potential influence of these microRNAs on cell fate acquisition during differentiation has been overlooked. Here, we show that miR-290-295 regulate the propensity of mESCs to acquire specific fates. We generated a new miR-290-295-null mESC model, which exhibits increased propensity to generate ectoderm, at the expense of endoderm and mesoderm lineages. We further found that in wild-type cells, miR-290-295 repress Pax6 and ectoderm differentiation; accordingly, Pax6 knockdown partially rescues the mESCs differentiation impairment that is caused by loss of miR-290-295. Thus, in addition to regulating self-renewal, the large reservoir of miR-290-295 in undifferentiated mESCs fine-tunes the expression of master transcriptional factors, such as Pax6, thereby regulating the equilibrium of fate acquisition by mESC descendants. Stem Cells 2013;31:2266-2272",
author = "Haggai Kaspi and Elik Chapnik and Maayan Levy and Gilad Beck and Eran Hornstein and Yoav Soen",
note = "We thank Rebecca Haffner‐Krausz and Golda Damari for mouse micromanipulation; Dr. Yael Fried and Dr. Elena Ainbinder (Stem Cell Core Facility Unit, Weizmann Institute of Science, Israel) for support in the ESC work; Inbal Zaltsman for assistance with quantitative polymerase chain reaction experiments; Dr. Jacob Hanna for critical reading of the manuscript. This work was supported by grants from the Israel Science Foundation (grant no. 730/12 to E.H. and 1541/09 to Y.S.) and Helen and Martin Kimmel Institute for Stem Cell Research. We also acknowledge support of the Leona M. and Harry B. Helmsley Charitable Trust (to Y.S.) and from Carolito Stiftung, Y. Leon Benoziyo Institute for Molecular Medicine, Charlene Vener New Scientist Fund, Estates of Fannie Sherr, Lola Asseof, Nathan Baltor, Maria Halphen, Julius and Ray Charlestein Foundation and the Fraida Foundation (to E.H.). E.H. is the incumbent of the Dr. Sydney Brenner Chair and the Helen and Milton A. Kimmelman Career Development Chair. Y.S. is the incumbent of Daniel E. Koshland Sr. Career Development Chair at the Weizmann Institute.",
year = "2013",
month = oct,
doi = "10.1002/stem.1465",
language = "الإنجليزيّة",
volume = "31",
pages = "2266--2272",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "Oxford University Press",
number = "10",
}