@inbook{dc88b77881874cec8ef03a1e0fa1a4cb,
title = "Bone Marrow–Derived Macrophage (BMDM ) Infection by Listeria monocytogenes",
abstract = "Listeria monocytogenes is a gram-positive bacterium adapted to life as both an environmental saprophyte and a pathogenic parasite of mammalian hosts, with a transcriptomic program tailored for each niche. Study of the L. monocytogenes pathogenic lifestyle requires conditions that mimic the mammalian niche. Of the myriad experimental models used to achieve such conditions, the bone marrow–derived macrophage (BMDM) is a relatively simple and reliable primary immune cell model for L. monocytogenes infections. Here we describe the extraction, preparation, and storage of BMDMs and their use in L. monocytogenes infection experiments.",
keywords = "Bacterial infection, Bone marrow derived macrophages, Intracellular growth, Listeria monocytogenes",
author = "Etai Boichis and {Ran Sapir}, Shai and Herskovits, {Anat A.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-1971-1_8",
language = "الإنجليزيّة",
series = "Methods in Molecular Biology",
pages = "83--93",
booktitle = "Methods in Molecular Biology",
}