BIPS - A code base for designing and coding of a Phage ImmunoPrecipitation Oligo Library

Sigal Leviatan; Iris N. Kalka; Thomas Vogl; Shelley Klompas; Adina Weinberger; Eran Segal

doi:10.1371/journal.pcbi.1010663

BIPS - A code base for designing and coding of a Phage ImmunoPrecipitation Oligo Library

Sigal Leviatan, Iris N. Kalka, Thomas Vogl, Shelley Klompas, Adina Weinberger, Eran Segal

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

BIPS (Build Phage ImmunoPrecipitation Sequencing library) is a software that converts a list of proteins into a custom DNA oligonucleotide library for the PhIP-Seq system. The tool creates constant-length oligonucleotides with internal barcodes, while maintaining the original length of the peptide. This allows using large libraries, of hundreds of thousands of oligonucleotides, while saving on the costs of sequencing and maintaining the accuracy of oligonucleotide reads identification. BIPS is available under GNU public license from: https://github.com/kalkairis/BuildPhIPSeqLibrary .

Original language	English
Article number	e1010663
Journal	PLoS Computational Biology
Volume	18
Issue number	11
DOIs	https://doi.org/10.1371/journal.pcbi.1010663
State	Published - Nov 2022

All Science Journal Classification (ASJC) codes

Ecology, Evolution, Behavior and Systematics
Modelling and Simulation
Ecology
Molecular Biology
Genetics
Cellular and Molecular Neuroscience
Computational Theory and Mathematics

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es_PLosComputBiol_BIPSaCodeBaseforDesigning_PV_2022Final published version, 1.26 MBLicense: CC BY

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title = "BIPS - A code base for designing and coding of a Phage ImmunoPrecipitation Oligo Library",

abstract = "BIPS (Build Phage ImmunoPrecipitation Sequencing library) is a software that converts a list of proteins into a custom DNA oligonucleotide library for the PhIP-Seq system. The tool creates constant-length oligonucleotides with internal barcodes, while maintaining the original length of the peptide. This allows using large libraries, of hundreds of thousands of oligonucleotides, while saving on the costs of sequencing and maintaining the accuracy of oligonucleotide reads identification. BIPS is available under GNU public license from: https://github.com/kalkairis/BuildPhIPSeqLibrary .",

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AU - Klompas, Shelley

AU - Weinberger, Adina

AU - Segal, Eran

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BIPS - A code base for designing and coding of a Phage ImmunoPrecipitation Oligo Library

Abstract

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