Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

Maxim Itkin; U. Heinig; Oren Tzfadia; A. J. Bhide; Belkrishna Ankush Shinde; P. D. Cardenas; S. E. Bocobza; Tamar Unger; Sergey Malitsky; R. Finkers; Y. Tikunov; A. Bovy; Yujana Rohidas Chikate; P. Singh; Ilana Rogachev; J. Beekwilder; A. P. Giri; Asaph Aharoni

doi:10.1126/science.1240230

Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

Maxim Itkin, U. Heinig, Oren Tzfadia, A. J. Bhide, Belkrishna Ankush Shinde, P. D. Cardenas, S. E. Bocobza, Tamar Unger, Sergey Malitsky, R. Finkers, Y. Tikunov, A. Bovy, Yujana Rohidas Chikate, P. Singh, Ilana Rogachev, J. Beekwilder, A. P. Giri, Asaph Aharoni

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants - as, for example, potato - are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

Original language	English
Pages (from-to)	175-179
Number of pages	5
Journal	Science
Volume	341
Issue number	6142
DOIs	https://doi.org/10.1126/science.1240230
State	Published - 2013

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Itkin, M., Heinig, U., Tzfadia, O., Bhide, A. J., Shinde, B. A., Cardenas, P. D., Bocobza, S. E., Unger, T., Malitsky, S., Finkers, R., Tikunov, Y., Bovy, A., Chikate, Y. R., Singh, P., Rogachev, I., Beekwilder, J., Giri, A. P., & Aharoni, A. (2013). Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes. Science, 341(6142), 175-179. https://doi.org/10.1126/science.1240230

@article{949b7617a5e3477da9c7e0e3a815423a,

title = "Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes",

abstract = "Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants - as, for example, potato - are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.",

author = "Maxim Itkin and U. Heinig and Oren Tzfadia and Bhide, {A. J.} and Shinde, {Belkrishna Ankush} and Cardenas, {P. D.} and Bocobza, {S. E.} and Tamar Unger and Sergey Malitsky and R. Finkers and Y. Tikunov and A. Bovy and Chikate, {Yujana Rohidas} and P. Singh and Ilana Rogachev and J. Beekwilder and Giri, {A. P.} and Asaph Aharoni",

note = "European Research Council (SAMIT-FP7 program); European Union [FP7-KBBE-2009-3-245121-ATHENA]; Volkswagen Foundation, Hannover, Germany [AZ: I/82 754]; Council of Scientific and Industrial Research (India); University Grants Commission (India)We thank A. Tishbee and R. Kramer for operating the UPLC-qTOF-MS instrument and the European Research Council (SAMIT-FP7 program) for supporting the work in the A. A. laboratory. A. A. is the incumbent of the Peter J. Cohn Professorial Chair. J. B. was supported by the European Union 7th Frame Anthocyanin and Polyphenol Bioactives for Health Enhancement through Nutritional Advancement (ATHENA) Project (FP7-KBBE-2009-3-245121-ATHENA). U. H. was partially supported by fellowship AZ: I/82 754, Volkswagen Foundation, Hannover, Germany. We thank the Council of Scientific and Industrial Research (India) for support to A. P. G. (Raman Research Fellowship), A. J. B., Y.C., and P. S. (Research Fellowship) and the University Grants Commission (India) for supporting B. S. We also thank D. R. Nelson for assistance with CYP450 gene classification and R. Last for critically reading the manuscript. A. A. and M. I. are inventors on publication number WO2012095843 A1, submitted by Yeda Research and Development Co. Ltd, which covers the use of the GAME4 gene for generating low-alkaloid fruit and tubers.",

year = "2013",

doi = "10.1126/science.1240230",

language = "الإنجليزيّة",

volume = "341",

pages = "175--179",

journal = "Science",

issn = "0036-8075",

publisher = "American Association for the Advancement of Science",

number = "6142",

}

Itkin, M, Heinig, U, Tzfadia, O, Bhide, AJ, Shinde, BA, Cardenas, PD, Bocobza, SE, Unger, T, Malitsky, S, Finkers, R, Tikunov, Y, Bovy, A, Chikate, YR, Singh, P, Rogachev, I, Beekwilder, J, Giri, AP & Aharoni, A 2013, 'Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes', Science, vol. 341, no. 6142, pp. 175-179. https://doi.org/10.1126/science.1240230

TY - JOUR

T1 - Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

AU - Itkin, Maxim

AU - Heinig, U.

AU - Tzfadia, Oren

AU - Bhide, A. J.

AU - Shinde, Belkrishna Ankush

AU - Cardenas, P. D.

AU - Bocobza, S. E.

AU - Unger, Tamar

AU - Malitsky, Sergey

AU - Finkers, R.

AU - Tikunov, Y.

AU - Bovy, A.

AU - Chikate, Yujana Rohidas

AU - Singh, P.

AU - Rogachev, Ilana

AU - Beekwilder, J.

AU - Giri, A. P.

AU - Aharoni, Asaph

N1 - European Research Council (SAMIT-FP7 program); European Union [FP7-KBBE-2009-3-245121-ATHENA]; Volkswagen Foundation, Hannover, Germany [AZ: I/82 754]; Council of Scientific and Industrial Research (India); University Grants Commission (India)We thank A. Tishbee and R. Kramer for operating the UPLC-qTOF-MS instrument and the European Research Council (SAMIT-FP7 program) for supporting the work in the A. A. laboratory. A. A. is the incumbent of the Peter J. Cohn Professorial Chair. J. B. was supported by the European Union 7th Frame Anthocyanin and Polyphenol Bioactives for Health Enhancement through Nutritional Advancement (ATHENA) Project (FP7-KBBE-2009-3-245121-ATHENA). U. H. was partially supported by fellowship AZ: I/82 754, Volkswagen Foundation, Hannover, Germany. We thank the Council of Scientific and Industrial Research (India) for support to A. P. G. (Raman Research Fellowship), A. J. B., Y.C., and P. S. (Research Fellowship) and the University Grants Commission (India) for supporting B. S. We also thank D. R. Nelson for assistance with CYP450 gene classification and R. Last for critically reading the manuscript. A. A. and M. I. are inventors on publication number WO2012095843 A1, submitted by Yeda Research and Development Co. Ltd, which covers the use of the GAME4 gene for generating low-alkaloid fruit and tubers.

PY - 2013

Y1 - 2013

N2 - Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants - as, for example, potato - are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

AB - Steroidal glycoalkaloids (SGAs) such as a-solanine found in solanaceous food plants - as, for example, potato - are antinutritional factors for humans. Comparative coexpression analysis between tomato and potato coupled with chemical profiling revealed an array of 10 genes that partake in SGA biosynthesis. We discovered that six of them exist as a cluster on chromosome 7, whereas an additional two are adjacent in a duplicated genomic region on chromosome 12. Following systematic functional analysis, we suggest a revised SGA biosynthetic pathway starting from cholesterol up to the tetrasaccharide moiety linked to the tomato SGA aglycone. Silencing GLYCOALKALOID METABOLISM 4 prevented accumulation of SGAs in potato tubers and tomato fruit. This may provide a means for removal of unsafe, antinutritional substances present in these widely used food crops.

UR - http://www.scopus.com/inward/record.url?scp=84880273715&partnerID=8YFLogxK

U2 - 10.1126/science.1240230

DO - 10.1126/science.1240230

M3 - مقالة

SN - 0036-8075

VL - 341

SP - 175

EP - 179

JO - Science

JF - Science

IS - 6142

ER -

Biosynthesis of antinutritional alkaloids in solanaceous crops is mediated by clustered genes

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