Biosensor based on DNA directed immobilization of enzymes onto optically sensitive porous Si

Giorgi Shtenberg; Naama Massad-Ivanir; Oren Moscovitz; Sinem Engin; Michal Sharon; Ljiljana Fruk; Ester Segal

doi:10.1557/opl.2013.806

Biosensor based on DNA directed immobilization of enzymes onto optically sensitive porous Si

Giorgi Shtenberg
, Naama Massad-Ivanir
, Oren Moscovitz
, Sinem Engin
, Michal Sharon
, Ljiljana Fruk
, Ester Segal

Weizmann Institute of Science, Technion - Israel Institute of Technology

Research output: Contribution to journal › Conference article › peer-review

Abstract

Optical biosensor for monitoring proteolytic activity is constructed by DNA-directed immobilization of enzymes onto porous Silicon nanostructures. This sensor configuration allows both protease recycling and easy surface regeneration for subsequent biosensing analysis by means of mild dehybridization conditions. We demonstrate real-time analysis of minute quantities of proteases paving the way for substrate profiling and the identification of cleavage sites. The biosensor is compatible with common proteomic methods and allows for a successful downstream mass spectrometry analysis of the reaction products.

Original language	English
Journal	Materials Research Society Symposium Proceedings
Volume	1569
Early online date	1 Oct 2013
DOIs	https://doi.org/10.1557/opl.2013.806
State	Published - Dec 2013
Event	2013 MRS Spring Meeting - San Francisco, CA, United States Duration: 1 Apr 2013 → 5 Apr 2013

Keywords

Mass spectroscopy
Optical properties
Sensor

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1557/opl.2013.806

Cite this

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title = "Biosensor based on DNA directed immobilization of enzymes onto optically sensitive porous Si",

abstract = "Optical biosensor for monitoring proteolytic activity is constructed by DNA-directed immobilization of enzymes onto porous Silicon nanostructures. This sensor configuration allows both protease recycling and easy surface regeneration for subsequent biosensing analysis by means of mild dehybridization conditions. We demonstrate real-time analysis of minute quantities of proteases paving the way for substrate profiling and the identification of cleavage sites. The biosensor is compatible with common proteomic methods and allows for a successful downstream mass spectrometry analysis of the reaction products.",

keywords = "Mass spectroscopy, Optical properties, Sensor",

author = "Giorgi Shtenberg and Naama Massad-Ivanir and Oren Moscovitz and Sinem Engin and Michal Sharon and Ljiljana Fruk and Ester Segal",

note = "Funding Information: Shtenberg Giorgi 1 Massad-Ivanir Naama 2 Moscovitz Oren 3 Engin Sinem 4 Sharon Michal 3 Fruk Ljiljana 4 Segal Ester 2 5 1 The Inter-Departmental Program of Biotechnology , Technion {\textendash} Israel Institute of Technology , Haifa 32000 , Israel 2 Department of Biotechnology and Food Engineering , Technion {\textendash} Israel Institute of Technology , Haifa 32000 , Israel 3 Department of Biological Chemistry , Weizmann Institute of Science , Rehovot 76100 , Israel 4 Karlsruhe Institute of Technology , DFG {\textendash} Center for Functional Nanostructures , Karlsruhe 76131 , Germany 5 The Russell Berrie Nanotechnology Institute , Technion {\textendash} Israel Institute of Technology , Haifa 32000 , Israel 2013 1569 Symposium NN {\textendash} Advanced Materials for Biological and Biomedical Applications 195 200 Copyright {\textcopyright} Materials Research Society 2013 2013 Materials Research Society; 2013 MRS Spring Meeting ; Conference date: 01-04-2013 Through 05-04-2013",

year = "2013",

month = dec,

doi = "10.1557/opl.2013.806",

language = "English",

volume = "1569",

journal = "Materials Research Society Symposium Proceedings",

issn = "0272-9172",

publisher = "Springer Nature",

}

TY - JOUR

T1 - Biosensor based on DNA directed immobilization of enzymes onto optically sensitive porous Si

AU - Shtenberg, Giorgi

AU - Massad-Ivanir, Naama

AU - Moscovitz, Oren

AU - Engin, Sinem

AU - Sharon, Michal

AU - Fruk, Ljiljana

AU - Segal, Ester

N1 - Funding Information: Shtenberg Giorgi 1 Massad-Ivanir Naama 2 Moscovitz Oren 3 Engin Sinem 4 Sharon Michal 3 Fruk Ljiljana 4 Segal Ester 2 5 1 The Inter-Departmental Program of Biotechnology , Technion – Israel Institute of Technology , Haifa 32000 , Israel 2 Department of Biotechnology and Food Engineering , Technion – Israel Institute of Technology , Haifa 32000 , Israel 3 Department of Biological Chemistry , Weizmann Institute of Science , Rehovot 76100 , Israel 4 Karlsruhe Institute of Technology , DFG – Center for Functional Nanostructures , Karlsruhe 76131 , Germany 5 The Russell Berrie Nanotechnology Institute , Technion – Israel Institute of Technology , Haifa 32000 , Israel 2013 1569 Symposium NN – Advanced Materials for Biological and Biomedical Applications 195 200 Copyright © Materials Research Society 2013 2013 Materials Research Society

PY - 2013/12

Y1 - 2013/12

N2 - Optical biosensor for monitoring proteolytic activity is constructed by DNA-directed immobilization of enzymes onto porous Silicon nanostructures. This sensor configuration allows both protease recycling and easy surface regeneration for subsequent biosensing analysis by means of mild dehybridization conditions. We demonstrate real-time analysis of minute quantities of proteases paving the way for substrate profiling and the identification of cleavage sites. The biosensor is compatible with common proteomic methods and allows for a successful downstream mass spectrometry analysis of the reaction products.

AB - Optical biosensor for monitoring proteolytic activity is constructed by DNA-directed immobilization of enzymes onto porous Silicon nanostructures. This sensor configuration allows both protease recycling and easy surface regeneration for subsequent biosensing analysis by means of mild dehybridization conditions. We demonstrate real-time analysis of minute quantities of proteases paving the way for substrate profiling and the identification of cleavage sites. The biosensor is compatible with common proteomic methods and allows for a successful downstream mass spectrometry analysis of the reaction products.

KW - Mass spectroscopy

KW - Optical properties

KW - Sensor

UR - https://www.scopus.com/pages/publications/84898926686

U2 - 10.1557/opl.2013.806

DO - 10.1557/opl.2013.806

M3 - Conference article

SN - 0272-9172

VL - 1569

JO - Materials Research Society Symposium Proceedings

JF - Materials Research Society Symposium Proceedings

T2 - 2013 MRS Spring Meeting

Y2 - 1 April 2013 through 5 April 2013

ER -

Biosensor based on DNA directed immobilization of enzymes onto optically sensitive porous Si

Abstract

Keywords

ASJC Scopus subject areas

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