@article{283dab94b5764544bbb5cd142a2da77c,
title = "Biophysical constraints of optogenetic inhibition at presynaptic terminals",
abstract = "We investigated the efficacy of optogenetic inhibition at presynaptic terminals using halorhodopsin, archaerhodopsin and chloride-conducting channelrhodopsins. Precisely timed activation of both archaerhodopsin and halorhodpsin at presynaptic terminals attenuated evoked release. However, sustained archaerhodopsin activation was paradoxically associated with increased spontaneous release. Activation of chloride-conducting channelrhodopsins triggered neurotransmitter release upon light onset. Thus, the biophysical properties of presynaptic terminals dictate unique boundary conditions for optogenetic manipulation.",
author = "Mathias Mahn and Matthias Prigge and Shiri Ron and Rivka Levy and Ofer Yizhar",
note = "We thank M. Segal (Weizmann Institute of Science) for discussions and reagents. We thank R. Zwang for help with cloning. We thank Y. Ziv, L. Fenno, I. Lampl, R. Paz and the Yizhar laboratory members for comments on the manuscript. We acknowledge support (to O.Y.) from the Human Frontier Science Program, the I-CORE program of the Planning and Budgeting Committee and the Israel Science Foundation (I-CORE grant no. 51/11, ISF grant no. 1351/12), a European Research Council starting grant (ERC-2013-StG OptoNeuromod 337637), the Adelis Foundation, the Lord Sieff of Brimpton Memorial Fund and the Candice Appleton Family Trust. O.Y. is supported by the Gertrude and Philip Nollman Career Development Chair. Contributions -M.M. and O.Y. designed the study. M.M. performed all electrophysiology and imaging experiments. R.L. prepared neuronal cultures and viral vectors. M.P. and S.R. helped with cloning and viral injections. M.M. and O.Y. analyzed and interpreted the results and wrote the manuscript.",
year = "2016",
month = mar,
day = "29",
doi = "https://doi.org/10.1038/nn.4266",
language = "الإنجليزيّة",
volume = "19",
pages = "554--556",
journal = "Nature Neuroscience",
issn = "1097-6256",
publisher = "Nature Publishing Group",
number = "4",
}