Biophysical constraints of optogenetic inhibition at presynaptic terminals

Mathias Mahn, Matthias Prigge, Shiri Ron, Rivka Levy, Ofer Yizhar

Research output: Contribution to journalArticlepeer-review

Abstract

We investigated the efficacy of optogenetic inhibition at presynaptic terminals using halorhodopsin, archaerhodopsin and chloride-conducting channelrhodopsins. Precisely timed activation of both archaerhodopsin and halorhodpsin at presynaptic terminals attenuated evoked release. However, sustained archaerhodopsin activation was paradoxically associated with increased spontaneous release. Activation of chloride-conducting channelrhodopsins triggered neurotransmitter release upon light onset. Thus, the biophysical properties of presynaptic terminals dictate unique boundary conditions for optogenetic manipulation.

Original languageEnglish
Pages (from-to)554-556
Number of pages3
JournalNature Neuroscience
Volume19
Issue number4
DOIs
StatePublished - 29 Mar 2016

All Science Journal Classification (ASJC) codes

  • General Neuroscience

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