Bionanocomposite films from resilin-CBD bound to cellulose nanocrystals

Amit Rivkin, Tiffany Abitbol, Yuval Nevo, Ronen Verker, Shaul Lapidot, Anton Komarov, Stephen C. Veldhuis, Galit Zilberman, Meital Reches, Emily D. Cranston, Oded Shoseyov

Research output: Contribution to journalArticlepeer-review

Abstract

This research explores the properties of bionanocomposite films prepared by binding recombinant resilin-like protein (res) consisting of the exon 1 resilin sequence from Drosophila melanogaster engineered to include a cellulose binding domain (CBD), to cellulose nanocrystals (CNCs). The optimal binding of res-CBD to CNCs was 1:5 by mass, and the resulting res-CBD-CNCs remained colloidally stable in water. Res-CBD-CNCs were solvent cast into transparent, free-standing films, which were more hydrophobic than neat CNC films, with water contact angles of 70-80 compared to 35-40 for the latter. In contrast to the multi-domain orientation typical of chiral nematic CNC films, res-CBD-CNC and CBD-CNC films exhibited long-range, uniaxial orientation that was apparently driven by the CBD moiety. Glycerol was studied as an additive in the films to determine whether the addition of a wet component to solvate the recombinant protein improved the mechanical properties of the res-CBD-CNC films. In comparison to the other films, res-CBD-CNC films were more elastic with added glycerol, demonstrating a range of 0.5-5wt% (i.e., the films responded more elastically to a given strain and/or were less plastically deformed by a given mechanical load), but became less elastic with added glycerol between 0.5-5wt%. Overall, films made of res-CBD-CNCs plus 0.5wt% glycerol displayed improved mechanical properties compared to neat CNC films, and with an increase in toughness of 150% and in elasticity of 100%.

Original languageAmerican English
Pages (from-to)44-58
Number of pages15
JournalIndustrial Biotechnology
Volume11
Issue number1
DOIs
StatePublished - 1 Feb 2015

All Science Journal Classification (ASJC) codes

  • Biotechnology

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