TY - JOUR
T1 - Biomolecular magic-angle spinning solid-state NMR
T2 - Recent methods and applications
AU - Goldbourt, Amir
N1 - Funding Information: Research in our lab referred to by this review was supported by the Israel Science Foundation , by the Marie-Curie Reintegration Grant ( EU-FP7-IRG ), and by the National Institute for Psychobiology in Israel . Our NMR spectrometer was funded in part by the Tel Aviv Center for Nanosciences and Nanotechnology . I would like to thank Omri Morag and Gili Abramov for producing the TEM and spectral images appearing in the graphical abstract and for their work on filamentous phage. Anat Haimovitch and Dr. Uzi Eliav performed the research on inositol monophosphatase and Bosmat Refaeli worked on the induction of the Entner–Doudoroff pathway in E. coli for isotopic enrichment.
PY - 2013/8
Y1 - 2013/8
N2 - The link of structure and dynamics of biomolecules and their complexes to their function and to many cellular processes has driven the quest for their detailed characterization by a variety of biophysical techniques. Magic-angle spinning solid-state nuclear magnetic resonance spectroscopy provides detailed information on the structural properties of such systems and in particular contributes invaluable information on non-soluble, large molecular-weight and non-crystalline biomolecules. This review summarizes the recent progress that has been made in the characterization of macromolecular assemblies, viruses, membrane proteins, amyloid fibrils, protein aggregates and more by magic-angle spinning solid-state NMR.
AB - The link of structure and dynamics of biomolecules and their complexes to their function and to many cellular processes has driven the quest for their detailed characterization by a variety of biophysical techniques. Magic-angle spinning solid-state nuclear magnetic resonance spectroscopy provides detailed information on the structural properties of such systems and in particular contributes invaluable information on non-soluble, large molecular-weight and non-crystalline biomolecules. This review summarizes the recent progress that has been made in the characterization of macromolecular assemblies, viruses, membrane proteins, amyloid fibrils, protein aggregates and more by magic-angle spinning solid-state NMR.
UR - http://www.scopus.com/inward/record.url?scp=84880927826&partnerID=8YFLogxK
U2 - 10.1016/j.copbio.2013.02.010
DO - 10.1016/j.copbio.2013.02.010
M3 - مقالة مرجعية
SN - 0958-1669
VL - 24
SP - 705
EP - 715
JO - Current Opinion in Biotechnology
JF - Current Opinion in Biotechnology
IS - 4
ER -