Axonal transcription factors signal retrogradely in lesioned peripheral nerve

Keren Ben-Yaakov, Shachar Y. Dagan, Yael Segal-Ruder, Ophir Shalem, Deepika Vuppalanchi, Dianna E. Willis, Dmitry Yudin, Ida Rishal, Franziska Rother, Michael Bader, Armin Blesch, Yitzhak Pilpel, Jeffery L. Twiss, Mike Fainzilber

Research output: Contribution to journalArticlepeer-review

Abstract

Retrograde axonal injury signalling stimulates cell body responses in lesioned peripheral neurons. The involvement of importins in retrograde transport suggests that transcription factors (TFs) might be directly involved in axonal injury signalling. Here, we show that multiple TFs are found in axons and associate with dynein in axoplasm from injured nerve. Biochemical and functional validation for one TF family establishes that axonal STAT3 is locally translated and activated upon injury, and is transported retrogradely with dynein and importin α5 to modulate survival of peripheral sensory neurons after injury. Hence, retrograde transport of TFs from axonal lesion sites provides a direct link between axon and nucleus.

Original languageEnglish
Pages (from-to)1350-1363
Number of pages14
JournalEMBO Journal
Volume31
Issue number6
DOIs
StatePublished - 21 Mar 2012

All Science Journal Classification (ASJC) codes

  • General Immunology and Microbiology
  • General Biochemistry,Genetics and Molecular Biology
  • Molecular Biology
  • General Neuroscience

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