Automatic detection of cell divisions (mitosis) in live-imaging microscopy images using Convolutional Neural Networks

Anat Shkolyar, Amit Gefen, Dafna Benayahu, Hayit Greenspan

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We propose a semi-automated pipeline for the detection of possible cell divisions in live-imaging microscopy and the classification of these mitosis candidates using a Convolutional Neural Network (CNN). We use time-lapse images of NIH3T3 scratch assay cultures, extract patches around bright candidate regions that then undergo segmentation and binarization, followed by a classification of the binary patches into either containing or not containing cell division. The classification is performed by training a Convolutional Neural Network on a specially constructed database. We show strong results of AUC = 0.91 and F-score = 0.89, competitive with state-of-the-art methods in this field.

Original languageEnglish
Title of host publication2015 37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
PublisherInstitute of Electrical and Electronics Engineers Inc.
Pages743-746
Number of pages4
ISBN (Electronic)9781424492718
DOIs
StatePublished - 4 Nov 2015
Event37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015 - Milan, Italy
Duration: 25 Aug 201529 Aug 2015

Publication series

NameProceedings of the Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBS
Volume2015-November

Conference

Conference37th Annual International Conference of the IEEE Engineering in Medicine and Biology Society, EMBC 2015
Country/TerritoryItaly
CityMilan
Period25/08/1529/08/15

All Science Journal Classification (ASJC) codes

  • Signal Processing
  • Biomedical Engineering
  • Computer Vision and Pattern Recognition
  • Health Informatics

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