Assessing Changes in the Expression Levels of Cell Surface Proteins with a Turn-on Fluorescent Molecular Probe

Joydev Hatai; Pragati Kishore Prasad; Naama Lahav-Mankovski; Noa Oppenheimer-Low; Tamar Unger; Yael Fridmann Sirkis; Tali Dadosh; Leila Motiei; David Margulies

doi:10.1039/d0cc07095e

Assessing Changes in the Expression Levels of Cell Surface Proteins with a Turn-on Fluorescent Molecular Probe

Joydev Hatai, Pragati Kishore Prasad, Naama Lahav-Mankovski, Noa Oppenheimer-Low, Tamar Unger, Yael Fridmann Sirkis, Tali Dadosh, Leila Motiei, David Margulies

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in liveEscherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum.

Original language	English
Pages (from-to)	1875-1878
Number of pages	4
Journal	Chemical Communications
Volume	57
Issue number	15
DOIs	https://doi.org/10.1039/d0cc07095e
State	Published - 21 Feb 2021

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

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10.1039/d0cc07095e

Cite this

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title = "Assessing Changes in the Expression Levels of Cell Surface Proteins with a Turn-on Fluorescent Molecular Probe",

abstract = "Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in liveEscherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum.",

author = "Joydev Hatai and Prasad, \{Pragati Kishore\} and Naama Lahav-Mankovski and Noa Oppenheimer-Low and Tamar Unger and Sirkis, \{Yael Fridmann\} and Tali Dadosh and Leila Motiei and David Margulies",

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doi = "10.1039/d0cc07095e",

language = "الإنجليزيّة",

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T1 - Assessing Changes in the Expression Levels of Cell Surface Proteins with a Turn-on Fluorescent Molecular Probe

AU - Hatai, Joydev

AU - Prasad, Pragati Kishore

AU - Lahav-Mankovski, Naama

AU - Oppenheimer-Low, Noa

AU - Unger, Tamar

AU - Sirkis, Yael Fridmann

AU - Dadosh, Tali

AU - Motiei, Leila

AU - Margulies, David

PY - 2021/2/21

Y1 - 2021/2/21

N2 - Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in liveEscherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum.

AB - Tri-nitrilotriacetic acid (NTA)-based fluorescent probes were developed and used to image His-tagged-labelled outer membrane protein C (His-OmpC) in liveEscherichia coli. One of these probes was designed to light up upon binding, which provided the means to assess changes in the His-OmpC expression levels by taking a simple fluorescence spectrum.

U2 - 10.1039/d0cc07095e

DO - 10.1039/d0cc07095e

M3 - مقالة

C2 - 33427257

SN - 1359-7345

VL - 57

SP - 1875

EP - 1878

JO - Chemical Communications

JF - Chemical Communications

IS - 15

ER -

Assessing Changes in the Expression Levels of Cell Surface Proteins with a Turn-on Fluorescent Molecular Probe

Abstract

All Science Journal Classification (ASJC) codes

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