TY - JOUR
T1 - Appropriate Thiamin Pyrophosphate Levels Are Required for Acclimation to Changes in Photoperiod
AU - Rosado-Souza, Laise
AU - Proost, Sebastian
AU - Moulin, Michael
AU - Bergmann, Susan
AU - Bocobza, Samuel E.
AU - Aharoni, Asaph
AU - Fitzpatrick, Teresa B.
AU - Mutwil, Marek
AU - Fernie, Alisdair R.
AU - Obata, Toshihiro
N1 - We acknowledge the support provided by the Infrastructure Group Biophysics and Photosynthesis and Dr. Mark A. Schoettler (from the Max Planck Institute of Molecular Plant Physiology) for the chlorophyll a fluorescence measurements. This work was supported by the National Council for Scientific and Technological Development (CNPq), Brazil (scholarship 246374/2012-8 to L.R.-S.), the Deutsche Forschungsgemeinschaft (DFG) within the Collaborative Research Centers, Sonderforschungsbereich (grant TRR 175/1 to A.R.F.), the Swiss National Science Foundation (grants 31003A-141117/1 and 31003A_162555/1 to T.B.F.), as well as the University of Geneva. L.R.-S. performed the experiments; L.R.-S., S.P., and M.Mo. analyzed samples and data; S.B. provided technical assistance; S.E.B. and A.A. provided seed material; T.B.F. and M.Mu. contributed to writing and reviewed the manuscript; T.O. and A.R.F. designed and supervised the study; L.R.-S., T.O., and A.R.F. wrote the article, with contributions from all of the authors.
PY - 2019/5
Y1 - 2019/5
N2 - Thiamin pyrophosphate (TPP) is the active form of vitamin B-1 and works as an essential cofactor for enzymes in key metabolic pathways, such as the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway. Although its action as a coenzyme has been well documented, the roles of TPP in plant metabolism are still not fully understood. Here, we investigated the functions of TPP in the regulation of the metabolic networks during photoperiod transition using previously described Arabidopsis (Arabidopsis thaliana) riboswitch mutant plants, which accumulate thiamin vitamers. The results show that photosynthetic and metabolic phenotypes of TPP riboswitch mutants are photoperiod dependent. Additionally, the mutants are more distinct from control plants when plants are transferred from a short-day to a long-day photoperiod, suggesting that TPP also plays a role in metabolic acclimation to the photoperiod. Control plants showed changes in the amplitude of diurnal oscillation in the levels of metabolites, including glycine, maltose, and fumarate, following the photoperiod transition. Interestingly, many of these changes are not present in TPP riboswitch mutant plants, demonstrating their lack of metabolic flexibility. Our results also indicate a close relationship between photorespiration and the TCA cycle, as TPP riboswitch mutants accumulate less photorespiratory intermediates. This study shows the potential role of vitamin B-1 in the diurnal regulation of central carbon metabolism in plants and the importance of maintaining appropriate cellular levels of thiamin vitamers for the plant's metabolic flexibility and ability to acclimate to an altered photoperiod.
AB - Thiamin pyrophosphate (TPP) is the active form of vitamin B-1 and works as an essential cofactor for enzymes in key metabolic pathways, such as the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway. Although its action as a coenzyme has been well documented, the roles of TPP in plant metabolism are still not fully understood. Here, we investigated the functions of TPP in the regulation of the metabolic networks during photoperiod transition using previously described Arabidopsis (Arabidopsis thaliana) riboswitch mutant plants, which accumulate thiamin vitamers. The results show that photosynthetic and metabolic phenotypes of TPP riboswitch mutants are photoperiod dependent. Additionally, the mutants are more distinct from control plants when plants are transferred from a short-day to a long-day photoperiod, suggesting that TPP also plays a role in metabolic acclimation to the photoperiod. Control plants showed changes in the amplitude of diurnal oscillation in the levels of metabolites, including glycine, maltose, and fumarate, following the photoperiod transition. Interestingly, many of these changes are not present in TPP riboswitch mutant plants, demonstrating their lack of metabolic flexibility. Our results also indicate a close relationship between photorespiration and the TCA cycle, as TPP riboswitch mutants accumulate less photorespiratory intermediates. This study shows the potential role of vitamin B-1 in the diurnal regulation of central carbon metabolism in plants and the importance of maintaining appropriate cellular levels of thiamin vitamers for the plant's metabolic flexibility and ability to acclimate to an altered photoperiod.
UR - http://www.scopus.com/inward/record.url?scp=85065662358&partnerID=8YFLogxK
U2 - https://doi.org/10.1104/pp.18.01346
DO - https://doi.org/10.1104/pp.18.01346
M3 - مقالة
C2 - 30837347
SN - 0032-0889
VL - 180
SP - 185
EP - 197
JO - Plant Physiology
JF - Plant Physiology
IS - 1
ER -