Applications of flow cytometry for measurement of autophagy

Alik Demishtein; Ziv Porat; Zvulun Elazar; Elena Shvets

doi:https://doi.org/10.1016/j.ymeth.2014.12.020

Applications of flow cytometry for measurement of autophagy

Alik Demishtein, Ziv Porat, Zvulun Elazar, Elena Shvets

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Autophagy is a dynamic catabolic process that plays a major role in sequestering and recycling cellular components in multiple physiological and pathophysiological conditions. Despite recent progress in our understanding of the autophagic process there is still a shortage of robust methods for monitoring autophagy in live cells. Flow cytometry, a reliable and unbiased method for quantitative collection of data in a high-throughput manner, was recently utilized to monitor autophagic activity in live and fixed mammalian cells. In this article we summarize the advantages and potential pitfalls of the use of flow cytometry to study autophagy.

Original language	English
Pages (from-to)	87-95
Number of pages	9
Journal	Methods
Volume	75
Early online date	3 Jan 2015
DOIs	https://doi.org/10.1016/j.ymeth.2014.12.020
State	Published - 15 Mar 2015

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology
Molecular Biology

Access to Document

https://doi.org/10.1016/j.ymeth.2014.12.020

Cite this

@article{3fba732e45274555b1e0282a935fc066,

title = "Applications of flow cytometry for measurement of autophagy",

abstract = "Autophagy is a dynamic catabolic process that plays a major role in sequestering and recycling cellular components in multiple physiological and pathophysiological conditions. Despite recent progress in our understanding of the autophagic process there is still a shortage of robust methods for monitoring autophagy in live cells. Flow cytometry, a reliable and unbiased method for quantitative collection of data in a high-throughput manner, was recently utilized to monitor autophagic activity in live and fixed mammalian cells. In this article we summarize the advantages and potential pitfalls of the use of flow cytometry to study autophagy.",

author = "Alik Demishtein and Ziv Porat and Zvulun Elazar and Elena Shvets",

note = "Israel Science Foundation ISF [535/11]; German Israeli Foundation GIF [1129/157]; ISF Legacy Heritage Fund [1309/13]Z.E. is the incumbent of the Harold Korda Chair of Biology. We are grateful for funding from the Israel Science Foundation ISF (Grant #535/11), the German Israeli Foundation GIF (Grant #1129/157), and the ISF Legacy Heritage Fund (Grant #1309/13).",

year = "2015",

month = mar,

day = "15",

doi = "https://doi.org/10.1016/j.ymeth.2014.12.020",

language = "الإنجليزيّة",

volume = "75",

pages = "87--95",

journal = "Methods",

issn = "1046-2023",

publisher = "Academic Press Inc.",

}

TY - JOUR

T1 - Applications of flow cytometry for measurement of autophagy

AU - Demishtein, Alik

AU - Porat, Ziv

AU - Elazar, Zvulun

AU - Shvets, Elena

N1 - Israel Science Foundation ISF [535/11]; German Israeli Foundation GIF [1129/157]; ISF Legacy Heritage Fund [1309/13]Z.E. is the incumbent of the Harold Korda Chair of Biology. We are grateful for funding from the Israel Science Foundation ISF (Grant #535/11), the German Israeli Foundation GIF (Grant #1129/157), and the ISF Legacy Heritage Fund (Grant #1309/13).

PY - 2015/3/15

Y1 - 2015/3/15

N2 - Autophagy is a dynamic catabolic process that plays a major role in sequestering and recycling cellular components in multiple physiological and pathophysiological conditions. Despite recent progress in our understanding of the autophagic process there is still a shortage of robust methods for monitoring autophagy in live cells. Flow cytometry, a reliable and unbiased method for quantitative collection of data in a high-throughput manner, was recently utilized to monitor autophagic activity in live and fixed mammalian cells. In this article we summarize the advantages and potential pitfalls of the use of flow cytometry to study autophagy.

AB - Autophagy is a dynamic catabolic process that plays a major role in sequestering and recycling cellular components in multiple physiological and pathophysiological conditions. Despite recent progress in our understanding of the autophagic process there is still a shortage of robust methods for monitoring autophagy in live cells. Flow cytometry, a reliable and unbiased method for quantitative collection of data in a high-throughput manner, was recently utilized to monitor autophagic activity in live and fixed mammalian cells. In this article we summarize the advantages and potential pitfalls of the use of flow cytometry to study autophagy.

UR - http://www.scopus.com/inward/record.url?scp=84924407537&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.ymeth.2014.12.020

DO - https://doi.org/10.1016/j.ymeth.2014.12.020

M3 - مقالة

SN - 1046-2023

VL - 75

SP - 87

EP - 95

JO - Methods

JF - Methods

ER -

Applications of flow cytometry for measurement of autophagy

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this