Antiproliferative activity of steroidal saponins from Balanites aegyptiaca - An in vitro study

Elie Beit-Yannai; Shimon Ben-Shabat; Noa Goldschmidt; Bishnu P. Chapagain; Rui Hai Liu; Zeev Wiesman

doi:https://doi.org/10.1016/j.phytol.2010.11.004

Antiproliferative activity of steroidal saponins from Balanites aegyptiaca - An in vitro study

Elie Beit-Yannai, Shimon Ben-Shabat, Noa Goldschmidt, Bishnu P. Chapagain, Rui Hai Liu, Zeev Wiesman

Department of Biotechnology Engineering

Ben-Gurion University of the Negev

Research output: Contribution to journal › Article › peer-review

Abstract

Saponins are well known as plant stress-induced protective agents. Saponin compounds are also considered responsible for numerous pharmacological properties including anticarcinogenic activity. This paper evaluates the antiproliferative activity and mode of action of spirostane (SAP-1016 and SAP-884) and furostane (KE-1046 and KE-1064) saponins we have isolated from Balanites aegyptiaca Del. The compound SAP-1016 (3β-O-β-d- xylopyranosyl-(1-3)-β-d-glucopyranosyl-(1-4)-[α-l-rhamnopyranosyl-(1- 2)]-β-d-glucopyranoside) showed potent antiproliferative activity against MCF-7 human breast cancer cells and HT-29 human colon cancer cells, with IC ₅₀ values of 2.4 ± 0.35 and 3.3 ± 0.19 μM, respectively, compared with dioscin, one of the most potent cytotoxic spirostane saponins, with IC₅₀ values of 3.1 ± 0.39 and 4.9 ± 0.32 μM, respectively. Significant anti-proliferative activity of SAP-1016 was also observed compared to a well-known anticancer agent, cisplatin, against both MCF-7 human breast cancer cells and HT-29 human colon cancer cells. Additionally, significant selectivity of growth inhibition, between MCF-7 breast cancer cells and HFF normal cells, was detected with the furostane saponins. Treatments of HT-29 cells with 5 μM SAP-1016 for 24 h generated caspase-3 cleavage and therefore apoptosis activation. SAP-1016 also demonstrated reactive oxygen species (ROS) generation in both HT-29 and MCF-7 cancer cells in a time-dependent manner.

Original language	American English
Pages (from-to)	43-47
Number of pages	5
Journal	Phytochemistry Letters
Volume	4
Issue number	1
DOIs	https://doi.org/10.1016/j.phytol.2010.11.004
State	Published - 15 Mar 2011

Keywords

Antiproliferation
Apoptotic
Balanites aegyptiaca
Cancer
ROS
Steroidal saponins

All Science Journal Classification (ASJC) codes

Biotechnology
Biochemistry
Agronomy and Crop Science
Plant Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.1016/j.phytol.2010.11.004

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title = "Antiproliferative activity of steroidal saponins from Balanites aegyptiaca - An in vitro study",

abstract = "Saponins are well known as plant stress-induced protective agents. Saponin compounds are also considered responsible for numerous pharmacological properties including anticarcinogenic activity. This paper evaluates the antiproliferative activity and mode of action of spirostane (SAP-1016 and SAP-884) and furostane (KE-1046 and KE-1064) saponins we have isolated from Balanites aegyptiaca Del. The compound SAP-1016 (3β-O-β-d- xylopyranosyl-(1-3)-β-d-glucopyranosyl-(1-4)-[α-l-rhamnopyranosyl-(1- 2)]-β-d-glucopyranoside) showed potent antiproliferative activity against MCF-7 human breast cancer cells and HT-29 human colon cancer cells, with IC 50 values of 2.4 ± 0.35 and 3.3 ± 0.19 μM, respectively, compared with dioscin, one of the most potent cytotoxic spirostane saponins, with IC50 values of 3.1 ± 0.39 and 4.9 ± 0.32 μM, respectively. Significant anti-proliferative activity of SAP-1016 was also observed compared to a well-known anticancer agent, cisplatin, against both MCF-7 human breast cancer cells and HT-29 human colon cancer cells. Additionally, significant selectivity of growth inhibition, between MCF-7 breast cancer cells and HFF normal cells, was detected with the furostane saponins. Treatments of HT-29 cells with 5 μM SAP-1016 for 24 h generated caspase-3 cleavage and therefore apoptosis activation. SAP-1016 also demonstrated reactive oxygen species (ROS) generation in both HT-29 and MCF-7 cancer cells in a time-dependent manner.",

keywords = "Antiproliferation, Apoptotic, Balanites aegyptiaca, Cancer, ROS, Steroidal saponins",

author = "Elie Beit-Yannai and Shimon Ben-Shabat and Noa Goldschmidt and Chapagain, {Bishnu P.} and Liu, {Rui Hai} and Zeev Wiesman",

note = "Funding Information: This work was supported in part by special grant from Marvin Israelow-Goldman Fund .",

year = "2011",

month = mar,

day = "15",

doi = "https://doi.org/10.1016/j.phytol.2010.11.004",

language = "American English",

volume = "4",

pages = "43--47",

journal = "Phytochemistry Letters",

issn = "1874-3900",

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TY - JOUR

T1 - Antiproliferative activity of steroidal saponins from Balanites aegyptiaca - An in vitro study

AU - Beit-Yannai, Elie

AU - Ben-Shabat, Shimon

AU - Goldschmidt, Noa

AU - Chapagain, Bishnu P.

AU - Liu, Rui Hai

AU - Wiesman, Zeev

N1 - Funding Information: This work was supported in part by special grant from Marvin Israelow-Goldman Fund .

PY - 2011/3/15

Y1 - 2011/3/15

N2 - Saponins are well known as plant stress-induced protective agents. Saponin compounds are also considered responsible for numerous pharmacological properties including anticarcinogenic activity. This paper evaluates the antiproliferative activity and mode of action of spirostane (SAP-1016 and SAP-884) and furostane (KE-1046 and KE-1064) saponins we have isolated from Balanites aegyptiaca Del. The compound SAP-1016 (3β-O-β-d- xylopyranosyl-(1-3)-β-d-glucopyranosyl-(1-4)-[α-l-rhamnopyranosyl-(1- 2)]-β-d-glucopyranoside) showed potent antiproliferative activity against MCF-7 human breast cancer cells and HT-29 human colon cancer cells, with IC 50 values of 2.4 ± 0.35 and 3.3 ± 0.19 μM, respectively, compared with dioscin, one of the most potent cytotoxic spirostane saponins, with IC50 values of 3.1 ± 0.39 and 4.9 ± 0.32 μM, respectively. Significant anti-proliferative activity of SAP-1016 was also observed compared to a well-known anticancer agent, cisplatin, against both MCF-7 human breast cancer cells and HT-29 human colon cancer cells. Additionally, significant selectivity of growth inhibition, between MCF-7 breast cancer cells and HFF normal cells, was detected with the furostane saponins. Treatments of HT-29 cells with 5 μM SAP-1016 for 24 h generated caspase-3 cleavage and therefore apoptosis activation. SAP-1016 also demonstrated reactive oxygen species (ROS) generation in both HT-29 and MCF-7 cancer cells in a time-dependent manner.

AB - Saponins are well known as plant stress-induced protective agents. Saponin compounds are also considered responsible for numerous pharmacological properties including anticarcinogenic activity. This paper evaluates the antiproliferative activity and mode of action of spirostane (SAP-1016 and SAP-884) and furostane (KE-1046 and KE-1064) saponins we have isolated from Balanites aegyptiaca Del. The compound SAP-1016 (3β-O-β-d- xylopyranosyl-(1-3)-β-d-glucopyranosyl-(1-4)-[α-l-rhamnopyranosyl-(1- 2)]-β-d-glucopyranoside) showed potent antiproliferative activity against MCF-7 human breast cancer cells and HT-29 human colon cancer cells, with IC 50 values of 2.4 ± 0.35 and 3.3 ± 0.19 μM, respectively, compared with dioscin, one of the most potent cytotoxic spirostane saponins, with IC50 values of 3.1 ± 0.39 and 4.9 ± 0.32 μM, respectively. Significant anti-proliferative activity of SAP-1016 was also observed compared to a well-known anticancer agent, cisplatin, against both MCF-7 human breast cancer cells and HT-29 human colon cancer cells. Additionally, significant selectivity of growth inhibition, between MCF-7 breast cancer cells and HFF normal cells, was detected with the furostane saponins. Treatments of HT-29 cells with 5 μM SAP-1016 for 24 h generated caspase-3 cleavage and therefore apoptosis activation. SAP-1016 also demonstrated reactive oxygen species (ROS) generation in both HT-29 and MCF-7 cancer cells in a time-dependent manner.

KW - Antiproliferation

KW - Apoptotic

KW - Balanites aegyptiaca

KW - Cancer

KW - ROS

KW - Steroidal saponins

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U2 - https://doi.org/10.1016/j.phytol.2010.11.004

DO - https://doi.org/10.1016/j.phytol.2010.11.004

M3 - Article

SN - 1874-3900

VL - 4

SP - 43

EP - 47

JO - Phytochemistry Letters

JF - Phytochemistry Letters

IS - 1

ER -

Antiproliferative activity of steroidal saponins from Balanites aegyptiaca - An in vitro study

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

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