Antibody isolation from a human synthetic combinatorial and other libraries of single-chain antibodies

Almog Bitton, Limor Nahary, Itai Benhar

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Antibody libraries came into existence 25 years ago when the accumulating sequence data of immunoglobulin genes and the advent of the PCR technology made it possible to clone antibody gene repertoires. Phage display (most common) and additional display and screening technologies were applied to pan out desired binding specificities from antibody libraries. “Synthetic” or “semisynthetic” libraries are from naive—non-immunized source and considered to be a source for many different targets, including self-antigens. As other antibody discovery tools, phage display is not an off-the-shelf technology and not offered as a kit but rather requires experience and expertise for making it indeed very useful. Here we present application notes that expand the usefulness of antibody phage display as a very versatile and robust antibody discovery tool.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
Pages349-363
Number of pages15
DOIs
StatePublished - 2018

Publication series

NameMethods in Molecular Biology
Volume1701

Keywords

  • CDRs—complementarity determining regions
  • FR—variable frameworks region
  • Phage display
  • ScFv—single-chain variable fragment
  • Single-chain antibodies
  • Synthetic library
  • V—variable region of antibody heavy chain
  • V—variable region of antibody light chain

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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