Anti-SARS-CoV-2 antibodies elicited by COVID-19 mRNA vaccine exhibit a unique glycosylation pattern

Inbal Farkash; Tali Feferman; Noy Cohen-Saban; Yahel Avraham; David Morgenstern; Grace Mayuni; Natasha Barth; Yaniv Lustig; Liron Miller; Dror S. Shouval; Asaf Biber; Yishai Levin; Rony Dahan

doi:10.1016/j.celrep.2021.110114

Anti-SARS-CoV-2 antibodies elicited by COVID-19 mRNA vaccine exhibit a unique glycosylation pattern

Inbal Farkash, Tali Feferman, Noy Cohen-Saban, Yahel Avraham, David Morgenstern, Grace Mayuni, Natasha Barth, Yaniv Lustig, Liron Miller, Dror S. Shouval, Asaf Biber, Yishai Levin, Rony Dahan

Tel Aviv University, Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Messenger RNA-based vaccines against COVID-19 induce a robust anti-SARS-CoV-2 antibody response with potent viral neutralization activity. Antibody effector functions are determined by their constant region subclasses and by their glycosylation patterns, but their role in vaccine efficacy is unclear. Moreover, whether vaccination induces antibodies similar to those in patients with COVID-19 remains unknown. We analyze BNT162b2 vaccine-induced IgG subclass distribution and Fc glycosylation patterns and their potential to drive effector function via Fcγ receptors and complement pathways. We identify unique and dynamic pro-inflammatory Fc compositions that are distinct from those in patients with COVID-19 and convalescents. Vaccine-induced anti-Spike IgG is characterized by distinct Fab- and Fc-mediated functions between different age groups and in comparison to antibodies generated during natural viral infection. These data highlight the heterogeneity of Fc responses to SARS-CoV-2 infection and vaccination and suggest that they support long-lasting protection differently.

Original language	English
Article number	110114
Number of pages	18
Journal	Cell Reports
Volume	37
Issue number	11
Early online date	Nov 2021
DOIs	https://doi.org/10.1016/j.celrep.2021.110114
State	Published - 14 Dec 2021

Keywords

BNT162b2 mRNA vaccine
Fc-Glycosylation
Fcγ receptors
IgG glycosylation
IgG-Fc
SARS-CoV-2
antibodies
complement
effector function
immunity

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.celrep.2021.110114

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title = "Anti-SARS-CoV-2 antibodies elicited by COVID-19 mRNA vaccine exhibit a unique glycosylation pattern",

abstract = "Messenger RNA-based vaccines against COVID-19 induce a robust anti-SARS-CoV-2 antibody response with potent viral neutralization activity. Antibody effector functions are determined by their constant region subclasses and by their glycosylation patterns, but their role in vaccine efficacy is unclear. Moreover, whether vaccination induces antibodies similar to those in patients with COVID-19 remains unknown. We analyze BNT162b2 vaccine-induced IgG subclass distribution and Fc glycosylation patterns and their potential to drive effector function via Fcγ receptors and complement pathways. We identify unique and dynamic pro-inflammatory Fc compositions that are distinct from those in patients with COVID-19 and convalescents. Vaccine-induced anti-Spike IgG is characterized by distinct Fab- and Fc-mediated functions between different age groups and in comparison to antibodies generated during natural viral infection. These data highlight the heterogeneity of Fc responses to SARS-CoV-2 infection and vaccination and suggest that they support long-lasting protection differently.",

keywords = "BNT162b2 mRNA vaccine, Fc-Glycosylation, Fcγ receptors, IgG glycosylation, IgG-Fc, SARS-CoV-2, antibodies, complement, effector function, immunity",

author = "Inbal Farkash and Tali Feferman and Noy Cohen-Saban and Yahel Avraham and David Morgenstern and Grace Mayuni and Natasha Barth and Yaniv Lustig and Liron Miller and Shouval, {Dror S.} and Asaf Biber and Yishai Levin and Rony Dahan",

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doi = "10.1016/j.celrep.2021.110114",

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T1 - Anti-SARS-CoV-2 antibodies elicited by COVID-19 mRNA vaccine exhibit a unique glycosylation pattern

AU - Farkash, Inbal

AU - Feferman, Tali

AU - Cohen-Saban, Noy

AU - Avraham, Yahel

AU - Morgenstern, David

AU - Mayuni, Grace

AU - Barth, Natasha

AU - Lustig, Yaniv

AU - Miller, Liron

AU - Shouval, Dror S.

AU - Biber, Asaf

AU - Levin, Yishai

AU - Dahan, Rony

PY - 2021/12/14

Y1 - 2021/12/14

N2 - Messenger RNA-based vaccines against COVID-19 induce a robust anti-SARS-CoV-2 antibody response with potent viral neutralization activity. Antibody effector functions are determined by their constant region subclasses and by their glycosylation patterns, but their role in vaccine efficacy is unclear. Moreover, whether vaccination induces antibodies similar to those in patients with COVID-19 remains unknown. We analyze BNT162b2 vaccine-induced IgG subclass distribution and Fc glycosylation patterns and their potential to drive effector function via Fcγ receptors and complement pathways. We identify unique and dynamic pro-inflammatory Fc compositions that are distinct from those in patients with COVID-19 and convalescents. Vaccine-induced anti-Spike IgG is characterized by distinct Fab- and Fc-mediated functions between different age groups and in comparison to antibodies generated during natural viral infection. These data highlight the heterogeneity of Fc responses to SARS-CoV-2 infection and vaccination and suggest that they support long-lasting protection differently.

AB - Messenger RNA-based vaccines against COVID-19 induce a robust anti-SARS-CoV-2 antibody response with potent viral neutralization activity. Antibody effector functions are determined by their constant region subclasses and by their glycosylation patterns, but their role in vaccine efficacy is unclear. Moreover, whether vaccination induces antibodies similar to those in patients with COVID-19 remains unknown. We analyze BNT162b2 vaccine-induced IgG subclass distribution and Fc glycosylation patterns and their potential to drive effector function via Fcγ receptors and complement pathways. We identify unique and dynamic pro-inflammatory Fc compositions that are distinct from those in patients with COVID-19 and convalescents. Vaccine-induced anti-Spike IgG is characterized by distinct Fab- and Fc-mediated functions between different age groups and in comparison to antibodies generated during natural viral infection. These data highlight the heterogeneity of Fc responses to SARS-CoV-2 infection and vaccination and suggest that they support long-lasting protection differently.

KW - BNT162b2 mRNA vaccine

KW - Fc-Glycosylation

KW - Fcγ receptors

KW - IgG glycosylation

KW - IgG-Fc

KW - SARS-CoV-2

KW - antibodies

KW - complement

KW - effector function

KW - immunity

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DO - 10.1016/j.celrep.2021.110114

M3 - مقالة

C2 - 34883043

SN - 2211-1247

VL - 37

JO - Cell Reports

JF - Cell Reports

IS - 11

M1 - 110114

ER -

Anti-SARS-CoV-2 antibodies elicited by COVID-19 mRNA vaccine exhibit a unique glycosylation pattern

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

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