Anti-SARS-CoV-2 antibodies elicited by COVID-19 mRNA vaccine exhibit a unique glycosylation pattern

Tali Feferman, Noy Cohen-Saban, Yahel Avraham, David Morgenstern, Grace Mayuni, Natasha Barth, Yaniv Lustig, Dror S. Shouval, Asaf Biber, Ilya Kirgner, Yishai Levin, Rony Dahan

Research output: Contribution to journalArticlepeer-review


Messenger RNA-based vaccines against COVID-19 induce a robust anti-SARS-CoV-2 antibody response with potent viral neutralization activity. Antibody effector functions are determined by their constant region subclasses and by their glycosylation patterns, but their role in vaccine efficacy is unclear. Moreover, whether vaccination induces antibodies similar to those in patients with COVID-19 remains unknown. We analyze BNT162b2 vaccine-induced IgG subclass distribution and Fc glycosylation patterns and their potential to drive effector function via Fcγ receptors and complement pathways. We identify unique and dynamic pro-inflammatory Fc compositions that are distinct from those in patients with COVID-19 and convalescents. Vaccine-induced anti-Spike IgG is characterized by distinct Fab- and Fc-mediated functions between different age groups and in comparison to antibodies generated during natural viral infection. These data highlight the heterogeneity of Fc responses to SARS-CoV-2 infection and vaccination and suggest that they support long-lasting protection differently.

Original languageEnglish
Article number110114
Number of pages18
JournalCell Reports
Issue number11
Early online dateNov 2021
StatePublished - 14 Dec 2021


  • BNT162b2 mRNA vaccine
  • Fc-Glycosylation
  • Fcγ receptors
  • IgG glycosylation
  • IgG-Fc
  • SARS-CoV-2
  • antibodies
  • complement
  • effector function
  • immunity

All Science Journal Classification (ASJC) codes

  • General Biochemistry,Genetics and Molecular Biology


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