Analysis of Steroidal Alkaloids and Saponins in Solanaceae Plant Extracts Using UPLC-qTOF Mass Spectrometry

U Heinig, Asaph Aharoni

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Plants of the Solanaceae family are renowned for the production of cholesterol-derived steroidal glycosides, including the nitrogen containing glycoalkaloids and steroidal saponins. In this chapter we describe the use of UPLC (Ultra Performance Liquid Chromatography) coupled with qTOF (Quadrupole Time-of-Flight) mass spectrometry for profiling of these two large classes of semipolar metabolites. The presented method includes an optimized sample preparation protocol, a procedure for high resolution chromatographic separation and metabolite detection using the TOF mass spectrometer which provides high resolution and mass accuracy. A detailed description for non-targeted data analysis and a strategy for putative identification of steroidal glycosides from complex extracts based on interpretation of mass fragmentation patterns is also provided. The described methodology allows profiling and putative identification of multiple steroidal glycoside compounds from the assortment of Solanaceae species producing these molecules.
Original languageEnglish
Title of host publicationPlant Isoprenoids
Subtitle of host publicationMethods in Molecular Biology
PublisherHumana Press
Pages171-185
Number of pages15
ISBN (Electronic)978-1-4939-0606-2
ISBN (Print)978-1-4939-0606-2; 978-1-4939-0605-5
DOIs
StatePublished - 31 Mar 2014

Publication series

NameMethods in Molecular Biology

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