@inbook{67b3ca9046a044e0a2a0f3648449cc8f,
title = "Analysis of Steroidal Alkaloids and Saponins in Solanaceae Plant Extracts Using UPLC-qTOF Mass Spectrometry",
abstract = "Plants of the Solanaceae family are renowned for the production of cholesterol-derived steroidal glycosides, including the nitrogen containing glycoalkaloids and steroidal saponins. In this chapter we describe the use of UPLC (Ultra Performance Liquid Chromatography) coupled with qTOF (Quadrupole Time-of-Flight) mass spectrometry for profiling of these two large classes of semipolar metabolites. The presented method includes an optimized sample preparation protocol, a procedure for high resolution chromatographic separation and metabolite detection using the TOF mass spectrometer which provides high resolution and mass accuracy. A detailed description for non-targeted data analysis and a strategy for putative identification of steroidal glycosides from complex extracts based on interpretation of mass fragmentation patterns is also provided. The described methodology allows profiling and putative identification of multiple steroidal glycoside compounds from the assortment of Solanaceae species producing these molecules.",
author = "U Heinig and Asaph Aharoni",
note = "We thank A. Tishbee for operating the UPLC/qTOF instrument, I. Rogachev and S. Meir for assistance in XCMS analysis, mass spectra interpretation and putative assignment of alkaloids from potato and tomato and the European Research Council (SAMIT-FP7 program) for supporting the work in the A. A. laboratory. A. A. is an incumbent of the Peter J. Cohn Professorial chair.",
year = "2014",
month = mar,
day = "31",
doi = "10.1007/978-1-4939-0606-2_12",
language = "الإنجليزيّة",
isbn = "978-1-4939-0606-2; 978-1-4939-0605-5",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "171--185",
booktitle = "Plant Isoprenoids",
address = "الولايات المتّحدة",
}