Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Gregory K. Webster; Ashley Elliott; Arik Dahan; Jonathan M. Miller

doi:10.1039/c0ay00698j

Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Gregory K. Webster, Ashley Elliott, Arik Dahan, Jonathan M. Miller

Research output: Contribution to journal › Article › peer-review

Abstract

A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

Original language	American English
Pages (from-to)	742-744
Number of pages	3
Journal	Analytical Methods
Volume	3
Issue number	3
DOIs	https://doi.org/10.1039/c0ay00698j
State	Published - 1 Mar 2011

All Science Journal Classification (ASJC) codes

General Engineering
General Chemical Engineering
Analytical Chemistry

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10.1039/c0ay00698j

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title = "Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection",

abstract = "A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent{\textregistered} Diamond Hydride column.",

author = "Webster, {Gregory K.} and Ashley Elliott and Arik Dahan and Miller, {Jonathan M.}",

year = "2011",

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doi = "10.1039/c0ay00698j",

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publisher = "Royal Society of Chemistry",

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AU - Webster, Gregory K.

AU - Elliott, Ashley

AU - Dahan, Arik

AU - Miller, Jonathan M.

PY - 2011/3/1

Y1 - 2011/3/1

N2 - A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

AB - A rapid method for the analysis of polyethylene glycol (PEG 400) in perfusate samples is presented. Because PEG 400 lacks a strong chromophore in the UV range, detection was accomplished using evaporative light scattering detection (ELSD). In order to optimize the ELSD signal performance for a volatile mobile phase, the chromatographic separation was optimized using aqueous normal phase conditions on a Cogent® Diamond Hydride column.

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U2 - 10.1039/c0ay00698j

DO - 10.1039/c0ay00698j

M3 - Article

SN - 1759-9660

VL - 3

SP - 742

EP - 744

JO - Analytical Methods

JF - Analytical Methods

IS - 3

ER -

Analysis of PEG 400 in perfusate samples by aqueous normal phase (ANP) chromatography with evaporative light scattering detection

Abstract

All Science Journal Classification (ASJC) codes

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