Analysis of apocarotenoid volatiles from lettuce (Lactuca sativa) induced by insect herbivores and characterization of carotenoid cleavage dioxygenase gene

Michal Eldar-Liebreich, Bhagwat Nawade, Mosaab Yahyaa, Liora Shaltiel-Harpaz, Moshe Coll, Asaf Sadeh, Mwafaq Ibdah

Research output: Contribution to journalArticlepeer-review

Abstract

Plant apocarotenoids have been shown to have a diverse biological role in herbivore–plant interactions. Despite their importance, little is known about herbivores’ effect on apocarotenoid emissions in Lactuca sativa. In this study, we examined changes in apocarotenoid emissions in lettuce leaves after infestation by two insects, viz., Spodoptera littoralis larvae and Myzus persicae aphids. We found that β-ionone and β-cyclocitral showed higher concentrations than the other apocarotenoids, with a significant increase as per the intensity of infestation of both herbivore species. Furthermore, we performed functional characterization of Lactuca sativa carotenoid cleavage dioxygenase 1 (LsCCD1) genes. Three LsCCD1 genes were overexpressed in E. coli strains, and recombinant proteins were assayed for cleavage activity on an array of carotenoid substrates. The LsCCD1 protein cleaved β-carotene at the 9,10 (9′,10′) positions producing β-ionone. The transcript analysis of LsCCD1 genes revealed differential expression patterns under varying levels of herbivores’ infestation, but the results were inconsistent with the pattern of β-ionone concentrations. Our results suggest that LsCCD1 is involved in the production of β-ionone, but other regulatory factors might be involved in its induction in response to herbivory. These results provide new insights into apocarotenoid production in response to insect herbivory in lettuce.

Original languageAmerican English
Article number94
Journal3 Biotech
Volume13
Issue number3
DOIs
StatePublished - Mar 2023

Keywords

  • Apocarotenoid
  • Carotenoid cleavage dioxygenase
  • Insect herbivory
  • Lactuca sativa
  • β-Ionone

All Science Journal Classification (ASJC) codes

  • Environmental Science (miscellaneous)
  • Agricultural and Biological Sciences (miscellaneous)
  • Biotechnology

Fingerprint

Dive into the research topics of 'Analysis of apocarotenoid volatiles from lettuce (Lactuca sativa) induced by insect herbivores and characterization of carotenoid cleavage dioxygenase gene'. Together they form a unique fingerprint.

Cite this