An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria

Yulia Shenberger; Lada Gevorkyan-Airapetov; Melanie Hirsch; Lukas Hofmann; Sharon Ruthstein

doi:https://doi.org/10.1039/d3cc03047d

An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria

Yulia Shenberger, Lada Gevorkyan-Airapetov, Melanie Hirsch, Lukas Hofmann, Sharon Ruthstein

Bar-Ilan University

Research output: Contribution to journal › Article › peer-review

Abstract

EPR in-cell spin-labeling was applied to CueR in E. coli. The methodology employed a Cu(ii)-NTA complexed with dHis. High resolved in-cell distance distributions were obtained revealing minor differences between in vitro and in-cell data. This methodology allows study of structural changes of any protein in-cell, independent of size or cellular system.

Original language	English
Pages (from-to)	10524-10527
Number of pages	4
Journal	Chemical Communications
Volume	59
Issue number	70
DOIs	https://doi.org/10.1039/d3cc03047d
State	Published - 29 Aug 2023

All Science Journal Classification (ASJC) codes

Electronic, Optical and Magnetic Materials
General Chemistry
Ceramics and Composites
Metals and Alloys
Materials Chemistry
Surfaces, Coatings and Films
Catalysis

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https://doi.org/10.1039/d3cc03047d

Cite this

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title = "An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria",

abstract = "EPR in-cell spin-labeling was applied to CueR in E. coli. The methodology employed a Cu(ii)-NTA complexed with dHis. High resolved in-cell distance distributions were obtained revealing minor differences between in vitro and in-cell data. This methodology allows study of structural changes of any protein in-cell, independent of size or cellular system.",

author = "Yulia Shenberger and Lada Gevorkyan-Airapetov and Melanie Hirsch and Lukas Hofmann and Sharon Ruthstein",

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T1 - An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria

AU - Shenberger, Yulia

AU - Gevorkyan-Airapetov, Lada

AU - Hirsch, Melanie

AU - Hofmann, Lukas

AU - Ruthstein, Sharon

PY - 2023/8/29

Y1 - 2023/8/29

N2 - EPR in-cell spin-labeling was applied to CueR in E. coli. The methodology employed a Cu(ii)-NTA complexed with dHis. High resolved in-cell distance distributions were obtained revealing minor differences between in vitro and in-cell data. This methodology allows study of structural changes of any protein in-cell, independent of size or cellular system.

AB - EPR in-cell spin-labeling was applied to CueR in E. coli. The methodology employed a Cu(ii)-NTA complexed with dHis. High resolved in-cell distance distributions were obtained revealing minor differences between in vitro and in-cell data. This methodology allows study of structural changes of any protein in-cell, independent of size or cellular system.

UR - http://www.scopus.com/inward/record.url?scp=85168568812&partnerID=8YFLogxK

U2 - https://doi.org/10.1039/d3cc03047d

DO - https://doi.org/10.1039/d3cc03047d

M3 - مقالة

C2 - 37563959

SN - 1359-7345

VL - 59

SP - 10524

EP - 10527

JO - Chemical Communications

JF - Chemical Communications

IS - 70

ER -

An in-cell spin-labelling methodology provides structural information on cytoplasmic proteins in bacteria

Abstract

All Science Journal Classification (ASJC) codes

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