An Improved Rapid Mixing Device for Time-Resolved Electrospray Mass Spectrometry Measurements

Nicholas Zinck, Ann-Kathrin Stark, Derek J. Wilson, Michal Sharon

Research output: Contribution to journalArticlepeer-review


Time series data can provide valuable insight into the complexity of biological reactions. Such information can be obtained by mass-spectrometry-based approaches that measure presteady-state kinetics. These methods are based on a mixing device that rapidly mixes the reactants prior to the on-line mass measurement of the transient intermediate steps. Here, we describe an improved continuous-flow mixing apparatus for real-time electrospray mass spectrometry measurements. Our setup was designed to minimize metal-solution interfaces and provide a sheath flow of nitrogen gas for generating stable and continuous spray that consequently enhances the signal-to-noise ratio. Moreover, the device was planned to enable easy mounting onto a mass spectrometer replacing the commercial electrospray ionization source. We demonstrate the performance of our apparatus by monitoring the unfolding reaction of cytochrome C, yielding improved signal-to-noise ratio and reduced experimental repeat errors.
Original languageEnglish
Pages (from-to)109-114
Number of pages6
Issue number3
StatePublished - Jun 2014


Dive into the research topics of 'An Improved Rapid Mixing Device for Time-Resolved Electrospray Mass Spectrometry Measurements'. Together they form a unique fingerprint.

Cite this