An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development

Zeshuai Wang; Zhisheng Wu; Hao Wang; Ruoqing Feng; Guanlin Wang; Muxi Li; Shuang Yin Wang; Xiaoyan Chen; Yiyi Su; Jun Wang; Weiwen Zhang; Yuzhou Bao; Zhenwei Lan; Zhuo Song; Yiheng Wang; Xianyang Luo; Lingyu Zhao; Anli Hou; Shuye Tian; Hongliang Gao; Wenbin Miao; Yingyu Liu; Huilin Wang; Cui Yin; Zhi Liang Ji; Mingqian Feng; Hongkun Liu; Lianghui Diao; Ido Amit; Yun Chen; Yong Zeng; Florent Ginhoux; Xueqing Wu; Yuanfang Zhu; Hanjie Li

doi:https://doi.org/10.1016/j.cell.2023.08.019

An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development

Zeshuai Wang, Zhisheng Wu, Hao Wang, Ruoqing Feng, Guanlin Wang, Muxi Li, Shuang Yin Wang, Xiaoyan Chen, Yiyi Su, Jun Wang, Weiwen Zhang, Yuzhou Bao, Zhenwei Lan, Zhuo Song, Yiheng Wang, Xianyang Luo, Lingyu Zhao, Anli Hou, Shuye Tian, Hongliang GaoWenbin Miao, Yingyu Liu, Huilin Wang, Cui Yin, Zhi Liang Ji, Mingqian Feng, Hongkun Liu, Lianghui Diao, Ido Amit, Yun Chen, Yong Zeng, Florent Ginhoux, Xueqing Wu, Yuanfang Zhu, Hanjie Li

Department of Systems Immunology

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Macrophages are heterogeneous and play critical roles in development and disease, but their diversity, function, and specification remain inadequately understood during human development. We generated a single-cell RNA sequencing map of the dynamics of human macrophage specification from PCW 4–26 across 19 tissues. We identified a microglia-like population and a proangiogenic population in 15 macrophage subtypes. Microglia-like cells, molecularly and morphologically similar to microglia in the CNS, are present in the fetal epidermis, testicle, and heart. They are the major immune population in the early epidermis, exhibit a polarized distribution along the dorsal-lateral-ventral axis, and interact with neural crest cells, modulating their differentiation along the melanocyte lineage. Through spatial and differentiation trajectory analysis, we also showed that proangiogenic macrophages are perivascular across fetal organs and likely yolk-sac-derived as microglia. Our study provides a comprehensive map of the heterogeneity and developmental dynamics of human macrophages and unravels their diverse functions during development.

Original language	English
Pages (from-to)	4454-4471.e19
Number of pages	38
Journal	Cell
Volume	186
Issue number	20
DOIs	https://doi.org/10.1016/j.cell.2023.08.019
State	Published - 28 Sep 2023

All Science Journal Classification (ASJC) codes

General Biochemistry,Genetics and Molecular Biology

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Wang, Z., Wu, Z., Wang, H., Feng, R., Wang, G., Li, M., Wang, S. Y., Chen, X., Su, Y., Wang, J., Zhang, W., Bao, Y., Lan, Z., Song, Z., Wang, Y., Luo, X., Zhao, L., Hou, A., Tian, S., ... Li, H. (2023). An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development. Cell, 186(20), 4454-4471.e19. https://doi.org/10.1016/j.cell.2023.08.019

@article{dc4b795eb47b424e9315314f815ac54a,

title = "An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development",

abstract = "Macrophages are heterogeneous and play critical roles in development and disease, but their diversity, function, and specification remain inadequately understood during human development. We generated a single-cell RNA sequencing map of the dynamics of human macrophage specification from PCW 4–26 across 19 tissues. We identified a microglia-like population and a proangiogenic population in 15 macrophage subtypes. Microglia-like cells, molecularly and morphologically similar to microglia in the CNS, are present in the fetal epidermis, testicle, and heart. They are the major immune population in the early epidermis, exhibit a polarized distribution along the dorsal-lateral-ventral axis, and interact with neural crest cells, modulating their differentiation along the melanocyte lineage. Through spatial and differentiation trajectory analysis, we also showed that proangiogenic macrophages are perivascular across fetal organs and likely yolk-sac-derived as microglia. Our study provides a comprehensive map of the heterogeneity and developmental dynamics of human macrophages and unravels their diverse functions during development.",

author = "Zeshuai Wang and Zhisheng Wu and Hao Wang and Ruoqing Feng and Guanlin Wang and Muxi Li and Wang, {Shuang Yin} and Xiaoyan Chen and Yiyi Su and Jun Wang and Weiwen Zhang and Yuzhou Bao and Zhenwei Lan and Zhuo Song and Yiheng Wang and Xianyang Luo and Lingyu Zhao and Anli Hou and Shuye Tian and Hongliang Gao and Wenbin Miao and Yingyu Liu and Huilin Wang and Cui Yin and Ji, {Zhi Liang} and Mingqian Feng and Hongkun Liu and Lianghui Diao and Ido Amit and Yun Chen and Yong Zeng and Florent Ginhoux and Xueqing Wu and Yuanfang Zhu and Hanjie Li",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier Inc.",

year = "2023",

month = sep,

day = "28",

doi = "https://doi.org/10.1016/j.cell.2023.08.019",

language = "الإنجليزيّة",

volume = "186",

pages = "4454--4471.e19",

journal = "Cell",

issn = "0092-8674",

publisher = "Cell Press",

number = "20",

}

Wang, Z, Wu, Z, Wang, H, Feng, R, Wang, G, Li, M, Wang, SY, Chen, X, Su, Y, Wang, J, Zhang, W, Bao, Y, Lan, Z, Song, Z, Wang, Y, Luo, X, Zhao, L, Hou, A, Tian, S, Gao, H, Miao, W, Liu, Y, Wang, H, Yin, C, Ji, ZL, Feng, M, Liu, H, Diao, L, Amit, I, Chen, Y, Zeng, Y, Ginhoux, F, Wu, X, Zhu, Y & Li, H 2023, 'An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development', Cell, vol. 186, no. 20, pp. 4454-4471.e19. https://doi.org/10.1016/j.cell.2023.08.019

TY - JOUR

T1 - An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development

AU - Wang, Zeshuai

AU - Wu, Zhisheng

AU - Wang, Hao

AU - Feng, Ruoqing

AU - Wang, Guanlin

AU - Li, Muxi

AU - Wang, Shuang Yin

AU - Chen, Xiaoyan

AU - Su, Yiyi

AU - Wang, Jun

AU - Zhang, Weiwen

AU - Bao, Yuzhou

AU - Lan, Zhenwei

AU - Song, Zhuo

AU - Wang, Yiheng

AU - Luo, Xianyang

AU - Zhao, Lingyu

AU - Hou, Anli

AU - Tian, Shuye

AU - Gao, Hongliang

AU - Miao, Wenbin

AU - Liu, Yingyu

AU - Wang, Huilin

AU - Yin, Cui

AU - Ji, Zhi Liang

AU - Feng, Mingqian

AU - Liu, Hongkun

AU - Diao, Lianghui

AU - Amit, Ido

AU - Chen, Yun

AU - Zeng, Yong

AU - Ginhoux, Florent

AU - Wu, Xueqing

AU - Zhu, Yuanfang

AU - Li, Hanjie

PY - 2023/9/28

Y1 - 2023/9/28

N2 - Macrophages are heterogeneous and play critical roles in development and disease, but their diversity, function, and specification remain inadequately understood during human development. We generated a single-cell RNA sequencing map of the dynamics of human macrophage specification from PCW 4–26 across 19 tissues. We identified a microglia-like population and a proangiogenic population in 15 macrophage subtypes. Microglia-like cells, molecularly and morphologically similar to microglia in the CNS, are present in the fetal epidermis, testicle, and heart. They are the major immune population in the early epidermis, exhibit a polarized distribution along the dorsal-lateral-ventral axis, and interact with neural crest cells, modulating their differentiation along the melanocyte lineage. Through spatial and differentiation trajectory analysis, we also showed that proangiogenic macrophages are perivascular across fetal organs and likely yolk-sac-derived as microglia. Our study provides a comprehensive map of the heterogeneity and developmental dynamics of human macrophages and unravels their diverse functions during development.

AB - Macrophages are heterogeneous and play critical roles in development and disease, but their diversity, function, and specification remain inadequately understood during human development. We generated a single-cell RNA sequencing map of the dynamics of human macrophage specification from PCW 4–26 across 19 tissues. We identified a microglia-like population and a proangiogenic population in 15 macrophage subtypes. Microglia-like cells, molecularly and morphologically similar to microglia in the CNS, are present in the fetal epidermis, testicle, and heart. They are the major immune population in the early epidermis, exhibit a polarized distribution along the dorsal-lateral-ventral axis, and interact with neural crest cells, modulating their differentiation along the melanocyte lineage. Through spatial and differentiation trajectory analysis, we also showed that proangiogenic macrophages are perivascular across fetal organs and likely yolk-sac-derived as microglia. Our study provides a comprehensive map of the heterogeneity and developmental dynamics of human macrophages and unravels their diverse functions during development.

UR - http://www.scopus.com/inward/record.url?scp=85172195114&partnerID=8YFLogxK

U2 - https://doi.org/10.1016/j.cell.2023.08.019

DO - https://doi.org/10.1016/j.cell.2023.08.019

M3 - مقالة

C2 - 37703875

SN - 0092-8674

VL - 186

SP - 4454-4471.e19

JO - Cell

JF - Cell

IS - 20

ER -

An immune cell atlas reveals the dynamics of human macrophage specification during prenatal development

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