TY - JOUR
T1 - An atlas of B-cell clonal distribution in the human body
AU - Meng, Wenzhao
AU - Zhang, Bochao
AU - Schwartz, Gregory W.
AU - Rosenfeld, Aaron M.
AU - Ren, Daqiu
AU - Thome, Joseph J.C.
AU - Carpenter, Dustin J.
AU - Matsuoka, Nobuhide
AU - Lerner, Harvey
AU - Friedman, Amy L.
AU - Granot, Tomer
AU - Farber, Donna L.
AU - Shlomchik, Mark J.
AU - Hershberg, Uri
AU - Luning Prak, Eline T.
N1 - Publisher Copyright: © 2017 Nature America, Inc.
PY - 2017/9/1
Y1 - 2017/9/1
N2 - B-cell responses result in clonal expansion, and can occur in a variety of tissues. To define how B-cell clones are distributed in the body, we sequenced 933,427 B-cell clonal lineages and mapped them to eight different anatomic compartments in six human organ donors. We show that large B-cell clones partition into two broad networks-one spans the blood, bone marrow, spleen and lung, while the other is restricted to tissues within the gastrointestinal (GI) tract (jejunum, ileum and colon). Notably, GI tract clones display extensive sharing of sequence variants among different portions of the tract and have higher frequencies of somatic hypermutation, suggesting extensive and serial rounds of clonal expansion and selection. Our findings provide an anatomic atlas of B-cell clonal lineages, their properties and tissue connections. This resource serves as a foundation for studies of tissue-based immunity, including vaccine responses, infections, autoimmunity and cancer.
AB - B-cell responses result in clonal expansion, and can occur in a variety of tissues. To define how B-cell clones are distributed in the body, we sequenced 933,427 B-cell clonal lineages and mapped them to eight different anatomic compartments in six human organ donors. We show that large B-cell clones partition into two broad networks-one spans the blood, bone marrow, spleen and lung, while the other is restricted to tissues within the gastrointestinal (GI) tract (jejunum, ileum and colon). Notably, GI tract clones display extensive sharing of sequence variants among different portions of the tract and have higher frequencies of somatic hypermutation, suggesting extensive and serial rounds of clonal expansion and selection. Our findings provide an anatomic atlas of B-cell clonal lineages, their properties and tissue connections. This resource serves as a foundation for studies of tissue-based immunity, including vaccine responses, infections, autoimmunity and cancer.
UR - http://www.scopus.com/inward/record.url?scp=85029311875&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/nbt.3942
DO - https://doi.org/10.1038/nbt.3942
M3 - Article
C2 - 28829438
SN - 1087-0156
VL - 35
SP - 879
EP - 886
JO - Nature biotechnology
JF - Nature biotechnology
IS - 9
ER -
