Amplification of highly mutated human Ig lambda light chains from an HIV-1 infected patient

Natalia T. Freund; Johannes F. Scheid; Hugo Mouquet; Michel C. Nussenzweig

doi:10.1016/j.jim.2015.01.011

Amplification of highly mutated human Ig lambda light chains from an HIV-1 infected patient

Natalia T. Freund, Johannes F. Scheid, Hugo Mouquet, Michel C. Nussenzweig

Research output: Contribution to journal › Article › peer-review

Abstract

Isolation and characterization of anti HIV-1 broadly neutralizing antibodies (bNAbs) have elucidated new epitopes and sites of viral vulnerability. Anti-HIV-1 bNAbs typically show high levels of somatic mutations in their variable region genes. This feature potentially limits antibody identification, since the mutated antibody sequences are no longer complimentary to primers designed based on germline antibody sequences. Here we report a new set of primers for Igλ light chains that aligns to the 5′ end of the leader sequence and is highly efficient for the amplification of antibodies that contain mutations and deletions in the 5′ end of human Igλ.

Original language	English
Pages (from-to)	61-65
Number of pages	5
Journal	Journal of Immunological Methods
Volume	418
DOIs	https://doi.org/10.1016/j.jim.2015.01.011
State	Published - 2015
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Anti-HIV-1 antibodies
Human Ig lambda
PCR
Single cell sorting
Somatic hypermutation

All Science Journal Classification (ASJC) codes

Immunology and Allergy
Immunology

Access to Document

10.1016/j.jim.2015.01.011

Cite this

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title = "Amplification of highly mutated human Ig lambda light chains from an HIV-1 infected patient",

abstract = "Isolation and characterization of anti HIV-1 broadly neutralizing antibodies (bNAbs) have elucidated new epitopes and sites of viral vulnerability. Anti-HIV-1 bNAbs typically show high levels of somatic mutations in their variable region genes. This feature potentially limits antibody identification, since the mutated antibody sequences are no longer complimentary to primers designed based on germline antibody sequences. Here we report a new set of primers for Igλ light chains that aligns to the 5′ end of the leader sequence and is highly efficient for the amplification of antibodies that contain mutations and deletions in the 5′ end of human Igλ.",

keywords = "Anti-HIV-1 antibodies, Human Ig lambda, PCR, Single cell sorting, Somatic hypermutation",

author = "Freund, {Natalia T.} and Scheid, {Johannes F.} and Hugo Mouquet and Nussenzweig, {Michel C.}",

note = "Publisher Copyright: {\textcopyright} 2015 Elsevier B.V.",

year = "2015",

doi = "10.1016/j.jim.2015.01.011",

language = "الإنجليزيّة",

volume = "418",

pages = "61--65",

journal = "Journal of Immunological Methods",

issn = "0022-1759",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Amplification of highly mutated human Ig lambda light chains from an HIV-1 infected patient

AU - Freund, Natalia T.

AU - Scheid, Johannes F.

AU - Mouquet, Hugo

AU - Nussenzweig, Michel C.

PY - 2015

Y1 - 2015

N2 - Isolation and characterization of anti HIV-1 broadly neutralizing antibodies (bNAbs) have elucidated new epitopes and sites of viral vulnerability. Anti-HIV-1 bNAbs typically show high levels of somatic mutations in their variable region genes. This feature potentially limits antibody identification, since the mutated antibody sequences are no longer complimentary to primers designed based on germline antibody sequences. Here we report a new set of primers for Igλ light chains that aligns to the 5′ end of the leader sequence and is highly efficient for the amplification of antibodies that contain mutations and deletions in the 5′ end of human Igλ.

AB - Isolation and characterization of anti HIV-1 broadly neutralizing antibodies (bNAbs) have elucidated new epitopes and sites of viral vulnerability. Anti-HIV-1 bNAbs typically show high levels of somatic mutations in their variable region genes. This feature potentially limits antibody identification, since the mutated antibody sequences are no longer complimentary to primers designed based on germline antibody sequences. Here we report a new set of primers for Igλ light chains that aligns to the 5′ end of the leader sequence and is highly efficient for the amplification of antibodies that contain mutations and deletions in the 5′ end of human Igλ.

KW - Anti-HIV-1 antibodies

KW - Human Ig lambda

KW - PCR

KW - Single cell sorting

KW - Somatic hypermutation

UR - http://www.scopus.com/inward/record.url?scp=84930360491&partnerID=8YFLogxK

U2 - 10.1016/j.jim.2015.01.011

DO - 10.1016/j.jim.2015.01.011

M3 - مقالة

C2 - 25667013

SN - 0022-1759

VL - 418

SP - 61

EP - 65

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

ER -

Amplification of highly mutated human Ig lambda light chains from an HIV-1 infected patient

Abstract

UN SDGs

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

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