Air-sampled filter analysis for endotoxins and DNA content

Naama Lang-Yona, Yinon Mazar, Michal Pardo, Yinon Rudich

Research output: Contribution to journalArticlepeer-review


Outdoor aerosol research commonly uses particulate matter sampled on filters. This procedure enables various characterizations of the collected particles to be performed in parallel. The purpose of the method presented here is to obtain a highly accurate and reliable analysis of the endotoxin and DNA content of bio-aerosols extracted from filters. The extraction of high molecular weight organic molecules, such as lipopolysaccharides, from sampled filters involves shaking the sample in a pyrogen-free water-based medium. The subsequent analysis is based on an enzymatic reaction that can be detected using a turbidimetric measurement. As a result of the high organic content on the sampled filters, the extraction of DNA from the samples is performed using a commercial DNA extraction kit that was originally designed for soils and modified to improve the DNA yield. The detection and quantification of specific microbial species using quantitative polymerase chain reaction (q-PCR) analysis are described and compared with other available methods.

Original languageEnglish
Article numbere53444
Number of pages11
JournalJournal of Visualized Experiments
Issue number109
StatePublished - 7 Mar 2016


  • Air samples
  • Bioaerosols
  • Cyanobacteria
  • Endotoxin
  • Environmental sciences
  • Filters
  • Issue 109
  • Marine aerosols
  • Q-PCR

All Science Journal Classification (ASJC) codes

  • General Chemical Engineering
  • General Immunology and Microbiology
  • General Biochemistry,Genetics and Molecular Biology
  • General Neuroscience


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