Adding to the CASeload: unwarranted p53 signaling induced by Cas9

Veronica Rendo; Oana M. Enache; Uri Ben-David

doi:https://doi.org/10.1080/23723556.2020.1789419

Adding to the CASeload: unwarranted p53 signaling induced by Cas9

Veronica Rendo, Oana M. Enache, Uri Ben-David

Human Molecular Genetics Biochemistry

Tel Aviv University

Research output: Contribution to journal › Comment/debate

Abstract

We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines. For a subset of cell lines with a wild-type tumor protein TP53 (best known as p53), we detected p53 pathway activation, DNA damage accumulation and emerging p53-inactivating mutations following Cas9 introduction. We discuss the potential implications of our findings in basic and translational research.

Original language	English
Article number	1789419
Journal	Molecular and Cellular Oncology
DOIs	https://doi.org/10.1080/23723556.2020.1789419
State	Published - 2020

Keywords

CRISPR
Cas9
DNA damage response
TP53 mutation
genome editing
p53 pathway

All Science Journal Classification (ASJC) codes

Molecular Medicine
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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https://doi.org/10.1080/23723556.2020.1789419

Cite this

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abstract = "We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines. For a subset of cell lines with a wild-type tumor protein TP53 (best known as p53), we detected p53 pathway activation, DNA damage accumulation and emerging p53-inactivating mutations following Cas9 introduction. We discuss the potential implications of our findings in basic and translational research.",

keywords = "CRISPR, Cas9, DNA damage response, TP53 mutation, genome editing, p53 pathway",

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year = "2020",

doi = "https://doi.org/10.1080/23723556.2020.1789419",

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AU - Enache, Oana M.

AU - Ben-David, Uri

PY - 2020

Y1 - 2020

N2 - We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines. For a subset of cell lines with a wild-type tumor protein TP53 (best known as p53), we detected p53 pathway activation, DNA damage accumulation and emerging p53-inactivating mutations following Cas9 introduction. We discuss the potential implications of our findings in basic and translational research.

AB - We investigated the genetic and transcriptional changes associated with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) associated protein 9 (Cas9) expression in human cancer cell lines. For a subset of cell lines with a wild-type tumor protein TP53 (best known as p53), we detected p53 pathway activation, DNA damage accumulation and emerging p53-inactivating mutations following Cas9 introduction. We discuss the potential implications of our findings in basic and translational research.

KW - CRISPR

KW - Cas9

KW - DNA damage response

KW - TP53 mutation

KW - genome editing

KW - p53 pathway

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M3 - تعليقَ / نقاش

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SN - 2372-3556

JO - Molecular and Cellular Oncology

JF - Molecular and Cellular Oncology

M1 - 1789419

ER -

Adding to the CASeload: unwarranted p53 signaling induced by Cas9

Abstract

Keywords

All Science Journal Classification (ASJC) codes

UN SDGs

Access to Document

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