TY - JOUR
T1 - ADAM metalloproteases promote a developmental switch in responsiveness to the axonal repellant Sema3A
AU - Romi, Erez
AU - Gokhman, Irena
AU - Wong, Eitan
AU - Antonovsky, Niv
AU - Ludwig, Andreas
AU - Sagi, Irit
AU - Saftig, Paul
AU - Tessier-Lavigne, Marc
AU - Yaron, Avraham
N1 - Minerva foundation; Deutsche Forschungsgemeinschaft DFG [SFB877-A3]; IUAP [P7/16]We are grateful to Keisuke Horiuchi and Carl P. Blobel for the ADAM17lox/lox line of mice. We thank Mike Fainzilber and Avraham Yaron's lab members for their critical review of the manuscript. This work was supported by the Minerva foundation (A.Y), Deutsche Forschungsgemeinschaft DFG (SFB877-A3) and the IUAP P7/16 (P.S).
PY - 2014/6/5
Y1 - 2014/6/5
N2 - During embryonic development, axons can gain and lose sensitivity to guidance cues, and this flexibility is essential for the correct wiring of the nervous system. Yet, the underlying molecular mechanisms are largely unknown. Here we show that receptor cleavage by ADAM (A Disintegrin And Metalloprotease) metalloproteases promotes murine sensory axons loss of responsiveness to the chemorepellant Sema3A. Genetic ablation of ADAM10 and ADAM17 disrupts the developmental downregulation of Neuropilin-1 (Nrp1), the receptor for Sema3A, in sensory axons. Moreover, this is correlated with gain of repulsive response to Sema3A. Overexpression of Nrp1 in neurons reverses axonal desensitization to Sema3A, but this is hampered in a mutant Nrp1 with high susceptibility to cleavage. Lastly, we detect guidance errors of proprioceptive axons in ADAM knockouts that are consistent with enhanced response to Sema3A. Our results provide the first evidence for involvement of ADAMs in regulating developmental switch in responsiveness to axonal guidance cues.
AB - During embryonic development, axons can gain and lose sensitivity to guidance cues, and this flexibility is essential for the correct wiring of the nervous system. Yet, the underlying molecular mechanisms are largely unknown. Here we show that receptor cleavage by ADAM (A Disintegrin And Metalloprotease) metalloproteases promotes murine sensory axons loss of responsiveness to the chemorepellant Sema3A. Genetic ablation of ADAM10 and ADAM17 disrupts the developmental downregulation of Neuropilin-1 (Nrp1), the receptor for Sema3A, in sensory axons. Moreover, this is correlated with gain of repulsive response to Sema3A. Overexpression of Nrp1 in neurons reverses axonal desensitization to Sema3A, but this is hampered in a mutant Nrp1 with high susceptibility to cleavage. Lastly, we detect guidance errors of proprioceptive axons in ADAM knockouts that are consistent with enhanced response to Sema3A. Our results provide the first evidence for involvement of ADAMs in regulating developmental switch in responsiveness to axonal guidance cues.
UR - http://www.scopus.com/inward/record.url?scp=84902128425&partnerID=8YFLogxK
U2 - https://doi.org/10.1038/ncomms5058
DO - https://doi.org/10.1038/ncomms5058
M3 - مقالة
SN - 2041-1723
VL - 5
JO - Nature Communications
JF - Nature Communications
M1 - 4058
ER -