TY - JOUR
T1 - Active diffusion positions the nucleus in mouse oocytes
AU - Almonacid, Maria
AU - Ahmed, Wylie W.
AU - Bussonnier, Matthias
AU - Mailly, Philippe
AU - Betz, Timo
AU - Voituriez, Raphael
AU - Gov, Nir
AU - Verlhac, Marie-Helene
N1 - Ligue Nationale contre le Cancer; Ligue Nationale Contre le Cancer [EL/2012/LNCC/MHV]; Fondation Bettencourt Schueller; program 'Investissements d'Avenir'; ANR [ANR-10-LABX-54 MEMO LIFE, ANR-11-IDEX-0001-02]; La Fondation Pierre-Gilles de Gennes; Marie Curie Actions; Axa PhD fellowship; French Agence Nationale de la Recherche [ANR-11-JSV5-0002]; ISF [500112]We thank J-Y. Tinevez (Institut Pasteur, Paris) for sharing the resources to TrackMate and MSD analysis, J. Unruh (Stowers Institute for Medical Research, Kansas City) tor sharing the resources tor STICS analysis, M. Schuh (MRC Cambridge) for providing the MyoVa and Vb tail plasmids and M-E. Terret for critical reading of the manuscript. M. Almonacid is a recipient of post-doctoral fellowship from the Ligue Nationale contre le Cancer. This work was supported by a grant from the Ligue Nationale Contre le Cancer (EL/2012/LNCC/MHV). This work has received support from the Fondation Bettencourt Schueller, and support under the program 'Investissements d'Avenir' launched by the French Government and implemented by the ANR, with the references: ANR-10-LABX-54 MEMO LIFE, ANR-11-IDEX-0001-02 PSL Research University. W.W.A. is a recipient of Postdoctoral fellowships from La Fondation Pierre-Gilles de Gennes and Marie Curie Actions. M.B. is a recipient of an Axa PhD fellowship. T.B. was supported by the French Agence Nationale de la Recherche (ANR-11-JSV5-0002). N.S.G. gratetully as financial support from the ISF (grant number 500112).
PY - 2015/4/30
Y1 - 2015/4/30
N2 - In somatic cells, the position of the cell centroid is dictated by the centrosome. The centrosome is instrumental in nucleus positioning, the two structures being physically connected. Mouse oocytes have no centrosomes, yet harbour centrally located nuclei. We demonstrate how oocytes define their geometric centre in the absence of centrosomes. Using live imaging of oocytes, knockout for the formin 2 actin nucleator, with off-centred nuclei, together with optical trapping and modelling, we discover an unprecedented mode of nucleus positioning. We document how active diffusion of actin-coated vesicles, driven by myosin Vb, generates a pressure gradient and a propulsion force sufficient to move the oocyte nucleus. It promotes fluidization of the cytoplasm, contributing to nucleus directional movement towards the centre. Our results highlight the potential of active diffusion, a prominent source of intracellular transport, able to move large organelles such as nuclei, providing in vivo evidence of its biological function.
AB - In somatic cells, the position of the cell centroid is dictated by the centrosome. The centrosome is instrumental in nucleus positioning, the two structures being physically connected. Mouse oocytes have no centrosomes, yet harbour centrally located nuclei. We demonstrate how oocytes define their geometric centre in the absence of centrosomes. Using live imaging of oocytes, knockout for the formin 2 actin nucleator, with off-centred nuclei, together with optical trapping and modelling, we discover an unprecedented mode of nucleus positioning. We document how active diffusion of actin-coated vesicles, driven by myosin Vb, generates a pressure gradient and a propulsion force sufficient to move the oocyte nucleus. It promotes fluidization of the cytoplasm, contributing to nucleus directional movement towards the centre. Our results highlight the potential of active diffusion, a prominent source of intracellular transport, able to move large organelles such as nuclei, providing in vivo evidence of its biological function.
UR - http://www.scopus.com/inward/record.url?scp=84925945177&partnerID=8YFLogxK
U2 - 10.1038/ncb3131
DO - 10.1038/ncb3131
M3 - مقالة
SN - 1465-7392
VL - 17
SP - 470
EP - 479
JO - Nature Cell Biology
JF - Nature Cell Biology
IS - 4
ER -