TY - JOUR
T1 - Active diffusion in oocytes nonspecifically centers large objects during prophase I and meiosis I
AU - Colin, Alexandra
AU - Letort, Gaelle
AU - Razin, Nitzan
AU - Almonacid, Maria
AU - Ahmed, Wylie
AU - Betz, Timo
AU - Terret, Marie-Emilie
AU - Gov, Nir S.
AU - Voituriez, Raphael
AU - Gueroui, Zoher
AU - Verlhac, Marie-Helene
N1 - A. Colin is supported by a Ministère de la Recherche doctoral fellowship. G. Letort is supported by the Agence Nationale de la Recherche (ANR-16-CE13 to M.-E. Terret). This work was supported by the Agence Nationale de la Recherche (ANR-DIVACEN to M.-H. Verlhac and R. Basto; Curie Institute, number 14-CE11), by a Fondation pour la Recherche Médicale Label (DEQ20150331758 to M.-H. Verlhac), by a Paris Sciences et Lettres Aux Frontière des Labex grant (MYOOCYTE, M.-H. Verlhac as coordinator), and by an Inca grant (PLBIO 2016-270-TRAN). This work has received support from the Fondation Bettencourt Schueller and support under the program Investissements d’Avenir launched by the French Government and implemented by the Agence Nationale de la Recherche, with the references ANR-10-LABX-54 MEMO LIFE and ANR-11-IDEX-0001-02 Paris Sciences et Lettres Research University. T. Betz is supported by the European Research Council (PolarizeMe, grant number CoG 771201). N.S. Gov is the incumbent of the Lee and William Abramowitz Professorial Chair of Biophysics and acknowledges support from the Israel Science Foundation (grant number 580/12). Author Contributions: R. Voituriez, Z. Gueroui, and M.-H. Verlhac supervised the work. M.-H. Verlhac did all the microinjections and imaging of mouse oocytes. A. Colin analyzed all experimental microinjection data. G. Letort did all the 3D simulations. N. Razin and N.S. Gov did the modeling. M. Almonacid and W. Ahmed performed the optical tweezers experiments under T. Betz’s supervision. W. Ahmed, T. Betz, N.S. Gov, and M.-E. Terret participated in the discussions on data interpretations together with A. Colin, G. Letort, R. Voituriez, Z. Gueroui, and M.-H. Verlhac. A. Colin, G. Letort, and M.-H. Verlhac wrote the manuscript, which was seen and corrected by all authors.
PY - 2020/3/2
Y1 - 2020/3/2
N2 - Nucleus centering in mouse oocytes results from a gradient of actin-positive vesicle activity and is essential for developmental success. Here, we analyze 3D model simulations to demonstrate how a gradient in the persistence of actin-positive vesicles can center objects of different sizes. We test model predictions by tracking the transport of exogenous passive tracers. The gradient of activity induces a centering force, akin to an effective pressure gradient, leading to the centering of oil droplets with velocities comparable to nuclear ones. Simulations and experimental measurements show that passive particles subjected to the gradient exhibit biased diffusion toward the center. Strikingly, we observe that the centering mechanism is maintained in meiosis I despite chromosome movement in the opposite direction; thus, it can counteract a process that specifically off-centers the spindle. In conclusion, our findings reconcile how common molecular players can participate in the two opposing functions of chromosome centering versus off-centering.
AB - Nucleus centering in mouse oocytes results from a gradient of actin-positive vesicle activity and is essential for developmental success. Here, we analyze 3D model simulations to demonstrate how a gradient in the persistence of actin-positive vesicles can center objects of different sizes. We test model predictions by tracking the transport of exogenous passive tracers. The gradient of activity induces a centering force, akin to an effective pressure gradient, leading to the centering of oil droplets with velocities comparable to nuclear ones. Simulations and experimental measurements show that passive particles subjected to the gradient exhibit biased diffusion toward the center. Strikingly, we observe that the centering mechanism is maintained in meiosis I despite chromosome movement in the opposite direction; thus, it can counteract a process that specifically off-centers the spindle. In conclusion, our findings reconcile how common molecular players can participate in the two opposing functions of chromosome centering versus off-centering.
U2 - https://doi.org/10.1083/jcb.201908195
DO - https://doi.org/10.1083/jcb.201908195
M3 - مقالة
C2 - 31952078
SN - 0021-9525
VL - 219
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 3
M1 - e201908195
ER -