A zinc selective oxytocin based biosensor

Evgeniy Mervinetsky; Israel Alshanski; Kiran Kumar Tadi; Mattan Hurevich; Shlomo Yitzchaik; Arezoo Dianat; Jörg Buchwald; Rafael Gutierrez; Gianaurelio Cuniberti

doi:https://doi.org/10.1039/c9tb01932d

A zinc selective oxytocin based biosensor

Evgeniy Mervinetsky, Israel Alshanski, Kiran Kumar Tadi, Mattan Hurevich, Shlomo Yitzchaik, Arezoo Dianat, Jörg Buchwald, Rafael Gutierrez, Gianaurelio Cuniberti

Institute of Chemistry

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Oxytocin is a peptide hormone with high affinity to both Zn²⁺ and Cu²⁺ ions compared to other metal ions. This affinity makes oxytocin an attractive recognition layer for monitoring the levels of these essential ions in biofluids. Native oxytocin cannot differentiate between Cu²⁺ and Zn²⁺ ions and hence it is not useful for sensing Zn²⁺ in the presence of Cu²⁺. We elucidated the effect of the terminal amine group of oxytocin on the affinity toward Cu²⁺ using theoretical calculations. We designed a new Zn²⁺ selective oxytocin-based biosensor that utilizes the terminal amine for surface anchoring, also preventing the response to Cu²⁺. The biosensor shows exceptional selectivity and very high sensitivity to Zn²⁺ in impedimetric biosensing.

Original language	English
Pages (from-to)	155-160
Number of pages	6
Journal	Journal of Materials Chemistry B
Volume	8
Issue number	1
DOIs	https://doi.org/10.1039/c9tb01932d
State	Published - 1 Jan 2020

All Science Journal Classification (ASJC) codes

General Chemistry
Biomedical Engineering
General Materials Science

Access to Document

https://doi.org/10.1039/c9tb01932d

Cite this

@article{04863e9a780541f69018b56a0f6d02a1,

title = "A zinc selective oxytocin based biosensor",

abstract = "Oxytocin is a peptide hormone with high affinity to both Zn2+ and Cu2+ ions compared to other metal ions. This affinity makes oxytocin an attractive recognition layer for monitoring the levels of these essential ions in biofluids. Native oxytocin cannot differentiate between Cu2+ and Zn2+ ions and hence it is not useful for sensing Zn2+ in the presence of Cu2+. We elucidated the effect of the terminal amine group of oxytocin on the affinity toward Cu2+ using theoretical calculations. We designed a new Zn2+ selective oxytocin-based biosensor that utilizes the terminal amine for surface anchoring, also preventing the response to Cu2+. The biosensor shows exceptional selectivity and very high sensitivity to Zn2+ in impedimetric biosensing.",

author = "Evgeniy Mervinetsky and Israel Alshanski and Tadi, {Kiran Kumar} and Mattan Hurevich and Shlomo Yitzchaik and Arezoo Dianat and J{\"o}rg Buchwald and Rafael Gutierrez and Gianaurelio Cuniberti",

note = "Publisher Copyright: This journal is {\textcopyright} The Royal Society of Chemistry.",

year = "2020",

month = jan,

day = "1",

doi = "https://doi.org/10.1039/c9tb01932d",

language = "الإنجليزيّة",

volume = "8",

pages = "155--160",

journal = "Journal of Materials Chemistry B",

issn = "2050-750X",

publisher = "Royal Society of Chemistry",

number = "1",

}

TY - JOUR

T1 - A zinc selective oxytocin based biosensor

AU - Mervinetsky, Evgeniy

AU - Alshanski, Israel

AU - Tadi, Kiran Kumar

AU - Hurevich, Mattan

AU - Yitzchaik, Shlomo

AU - Dianat, Arezoo

AU - Buchwald, Jörg

AU - Gutierrez, Rafael

AU - Cuniberti, Gianaurelio

PY - 2020/1/1

Y1 - 2020/1/1

N2 - Oxytocin is a peptide hormone with high affinity to both Zn2+ and Cu2+ ions compared to other metal ions. This affinity makes oxytocin an attractive recognition layer for monitoring the levels of these essential ions in biofluids. Native oxytocin cannot differentiate between Cu2+ and Zn2+ ions and hence it is not useful for sensing Zn2+ in the presence of Cu2+. We elucidated the effect of the terminal amine group of oxytocin on the affinity toward Cu2+ using theoretical calculations. We designed a new Zn2+ selective oxytocin-based biosensor that utilizes the terminal amine for surface anchoring, also preventing the response to Cu2+. The biosensor shows exceptional selectivity and very high sensitivity to Zn2+ in impedimetric biosensing.

AB - Oxytocin is a peptide hormone with high affinity to both Zn2+ and Cu2+ ions compared to other metal ions. This affinity makes oxytocin an attractive recognition layer for monitoring the levels of these essential ions in biofluids. Native oxytocin cannot differentiate between Cu2+ and Zn2+ ions and hence it is not useful for sensing Zn2+ in the presence of Cu2+. We elucidated the effect of the terminal amine group of oxytocin on the affinity toward Cu2+ using theoretical calculations. We designed a new Zn2+ selective oxytocin-based biosensor that utilizes the terminal amine for surface anchoring, also preventing the response to Cu2+. The biosensor shows exceptional selectivity and very high sensitivity to Zn2+ in impedimetric biosensing.

UR - http://www.scopus.com/inward/record.url?scp=85076876611&partnerID=8YFLogxK

U2 - https://doi.org/10.1039/c9tb01932d

DO - https://doi.org/10.1039/c9tb01932d

M3 - مقالة

C2 - 31782469

SN - 2050-750X

VL - 8

SP - 155

EP - 160

JO - Journal of Materials Chemistry B

JF - Journal of Materials Chemistry B

IS - 1

ER -

A zinc selective oxytocin based biosensor

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this