@article{05dee5feea0440858cab8a7bf6b80462,
title = "A versatile genome-scale PCR-based pipeline for high-definition DNA FISH",
abstract = "We developed a cost-effective genome-scale PCR-based method for high-definition DNA FISH (HD-FISH). We visualized gene loci with diffraction-limited resolution, chromosomes as spot clusters and single genes together with transcripts by combining HD-FISH with single-molecule RNA FISH. We provide a database of over 4.3 million primer pairs targeting the human and mouse genomes that is readily usable for rapid and flexible generation of probes.",
author = "Magda Bienko and Nicola Crosetto and Leonid Teytelman and Sandy Klemm and Shalev Itzkovitz and {Van Oudenaarden}, Alexander",
note = "US National Institutes of Health (NIH)/National Cancer Institute Physical Sciences Oncology Center at Massachusetts Institute of Technology [U54CA143874]; NIH [1DP10D003936]; Nederlandse Organisatie voor Wetenschappelijk Onderzoek Vici award; Human Frontiers Science Program We thank P. Junker and S. Semrau for helpful discussions. We are grateful to R.A. Weinberg (Massachusetts Institute of Technology) for providing hTERT-HME1 cells. This work was supported by the US National Institutes of Health (NIH)/National Cancer Institute Physical Sciences Oncology Center at Massachusetts Institute of Technology (U54CA143874), an NIH Pioneer award (1DP10D003936) and a Nederlandse Organisatie voor Wetenschappelijk Onderzoek Vici award to A.v.O. M.B. and S.I. are sponsored by the Human Frontiers Science Program.",
year = "2013",
month = feb,
doi = "https://doi.org/10.1038/nmeth.2306",
language = "الإنجليزيّة",
volume = "10",
pages = "122--124",
journal = "Nature Methods",
issn = "1548-7091",
publisher = "Nature Research",
number = "2",
}