A TILLING population for cucumber forward and reverse genetics

R Fraenkel; I. Kovalski; C. Troadec; A. Bendahmane; R. Perl-Treves; N. Sari; I. Solmaz; V. Aras

A TILLING population for cucumber forward and reverse genetics

R Fraenkel, I. Kovalski, C. Troadec, A. Bendahmane, R. Perl-Treves, N. Sari (Editor), I. Solmaz (Editor), V. Aras (Editor)

The Mina and Everard Goodman Faculty of Life Sciences

Bar-Ilan University

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › peer-review

Abstract

Ordered collections of mutants serve as invaluable tools in biology, and in functional genomics in particular. TILLING (targeting induced local lesions in genomes) provides an efficient method to study the possible phenotype controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber. We report the development of a TILLING cucumber population, prepared by EMS mutagenesis in the Poinsett-76 genetic background. The population was evaluated by screening morphological mutations, and its suitability for detecting single nucleotide polymorphism in selected genes has been tested. Such previously-unavailable asset in cucumber can be exploited for diverse breeding and research purposes

Original language	English
Title of host publication	Cucurbitaceae 2012. Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae
Publisher	Cukurova University, Faculty of Medicine
ISBN (Print)	9786056329708
State	Published - 2012

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Fraenkel, R., Kovalski, I., Troadec, C., Bendahmane, A., Perl-Treves, R., Sari, N. (Ed.), Solmaz, I. (Ed.), & Aras, V. (Ed.) (2012). A TILLING population for cucumber forward and reverse genetics. In Cucurbitaceae 2012. Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae Cukurova University, Faculty of Medicine. https://www.cabdirect.org/cabdirect/abstract/20133109878

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title = "A TILLING population for cucumber forward and reverse genetics",

abstract = "Ordered collections of mutants serve as invaluable tools in biology, and in functional genomics in particular. TILLING (targeting induced local lesions in genomes) provides an efficient method to study the possible phenotype controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber. We report the development of a TILLING cucumber population, prepared by EMS mutagenesis in the Poinsett-76 genetic background. The population was evaluated by screening morphological mutations, and its suitability for detecting single nucleotide polymorphism in selected genes has been tested. Such previously-unavailable asset in cucumber can be exploited for diverse breeding and research purposes",

author = "R Fraenkel and I. Kovalski and C. Troadec and A. Bendahmane and R. Perl-Treves and N. Sari and I. Solmaz and V. Aras",

note = "Place of conference:Turkey",

year = "2012",

language = "الإنجليزيّة",

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Fraenkel, R, Kovalski, I, Troadec, C, Bendahmane, A, Perl-Treves, R, Sari, N (ed.), Solmaz, I (ed.) & Aras, V (ed.) 2012, A TILLING population for cucumber forward and reverse genetics. in Cucurbitaceae 2012. Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae. Cukurova University, Faculty of Medicine. <https://www.cabdirect.org/cabdirect/abstract/20133109878>

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T1 - A TILLING population for cucumber forward and reverse genetics

AU - Fraenkel, R

AU - Kovalski, I.

AU - Troadec, C.

AU - Bendahmane, A.

AU - Perl-Treves, R.

A2 - Sari, N.

A2 - Solmaz, I.

A2 - Aras, V.

N1 - Place of conference:Turkey

PY - 2012

Y1 - 2012

N2 - Ordered collections of mutants serve as invaluable tools in biology, and in functional genomics in particular. TILLING (targeting induced local lesions in genomes) provides an efficient method to study the possible phenotype controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber. We report the development of a TILLING cucumber population, prepared by EMS mutagenesis in the Poinsett-76 genetic background. The population was evaluated by screening morphological mutations, and its suitability for detecting single nucleotide polymorphism in selected genes has been tested. Such previously-unavailable asset in cucumber can be exploited for diverse breeding and research purposes

AB - Ordered collections of mutants serve as invaluable tools in biology, and in functional genomics in particular. TILLING (targeting induced local lesions in genomes) provides an efficient method to study the possible phenotype controlled by gene sequences whose function is unknown. This method can replace transgenic techniques for the functional validation of cloned genes, especially in the case of transformation-recalcitrant plants such as cucumber. We report the development of a TILLING cucumber population, prepared by EMS mutagenesis in the Poinsett-76 genetic background. The population was evaluated by screening morphological mutations, and its suitability for detecting single nucleotide polymorphism in selected genes has been tested. Such previously-unavailable asset in cucumber can be exploited for diverse breeding and research purposes

UR - https://scholar.google.co.il/scholar?q=A+TILLING+population+for+cucumber+forward+and+reverse+genetics&btnG=&hl=en&as_sdt=0%2C5

M3 - منشور من مؤتمر

SN - 9786056329708

BT - Cucurbitaceae 2012. Proceedings of the Xth EUCARPIA Meeting on Genetics and Breeding of Cucurbitaceae

PB - Cukurova University, Faculty of Medicine

ER -

A TILLING population for cucumber forward and reverse genetics

Abstract

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