Abstract
This protocol describes the culturing of the nematode Caenorhabditis elegans (C. elegans) in a sterol-defined experimental system and the subsequent quantitative analysis of C. elegans sterols through gas chromatography-mass spectrometry. Although studied primarily in mammals, sterols are essential biomolecules for most eukaryotes. C. elegans cannot synthesize sterols and thus relies on the uptake of dietary sterols. Therefore, C. elegans is a powerful system to study metabolism in sterol-defined conditions that are described in our protocol. For complete details on the use and execution of this protocol, please refer to Shamsuzzama et al. (2020).
Original language | American English |
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Article number | 100710 |
Journal | STAR Protocols |
Volume | 2 |
Issue number | 3 |
DOIs | |
State | Published - 17 Sep 2021 |
Keywords
- Genetics
- Mass Spectrometry
- Metabolism
- Model Organisms
All Science Journal Classification (ASJC) codes
- General Immunology and Microbiology
- General Biochemistry,Genetics and Molecular Biology
- General Neuroscience