A simplified and efficient method for single-step, targeted gene deletion in mycobacteria

Yahav Bracha; Daniel Barkan

doi:10.1016/j.tube.2024.102520

A simplified and efficient method for single-step, targeted gene deletion in mycobacteria

Yahav Bracha, Daniel Barkan

The Koret School of Veterinary Medicine

The Hebrew University of Jerusalem

Research output: Contribution to journal › Article › peer-review

Abstract

Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.

Original language	English
Article number	102520
Journal	Tuberculosis
Volume	147
DOIs	https://doi.org/10.1016/j.tube.2024.102520
State	Published - Jul 2024

Keywords

Gene deletion
Genetic manipulation
Mycobacteria

All Science Journal Classification (ASJC) codes

Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

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10.1016/j.tube.2024.102520

Cite this

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title = "A simplified and efficient method for single-step, targeted gene deletion in mycobacteria",

abstract = "Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.",

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year = "2024",

month = jul,

doi = "10.1016/j.tube.2024.102520",

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AU - Bracha, Yahav

AU - Barkan, Daniel

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N2 - Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.

AB - Targeted gene deletion in mycobacteria remain complicated, requiring expertise and multiple steps. Here we present a single-step, easy to understand and perform method for targeted gene deletion. Using this method, we successfully deleted several genes in both M. smegmatis and M. abscessus. We believe this method will facilitate molecular research of mycobacteria and make it accessible to a greater number of researchers throughout the world.

KW - Gene deletion

KW - Genetic manipulation

KW - Mycobacteria

UR - http://www.scopus.com/inward/record.url?scp=85193510579&partnerID=8YFLogxK

U2 - 10.1016/j.tube.2024.102520

DO - 10.1016/j.tube.2024.102520

M3 - مقالة

C2 - 38781657

SN - 1472-9792

VL - 147

JO - Tuberculosis

JF - Tuberculosis

M1 - 102520

ER -

A simplified and efficient method for single-step, targeted gene deletion in mycobacteria

Abstract

Keywords

All Science Journal Classification (ASJC) codes

Access to Document

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