@article{d6b59253758d447ab1f2b67ad8323fa1,
title = "A secreted disulfide catalyst controls extracellular matrix composition and function",
abstract = "Disulfide bond formation in secretory proteins occurs primarily in the endoplasmic reticulum (ER), where multiple enzyme families catalyze cysteine cross-linking. Quiescin sulfhydryl oxidase 1 (QSOX1) is an atypical disulfide catalyst, localized to the Golgi apparatus or secreted from cells. We examined the physiological function for extracellular catalysis of de novo disulfide bond formation by QSOX1. QSOX1 activity was required for incorporation of laminin into the extracellular matrix (ECM) synthesized by fibroblasts, and ECM produced without QSOX1 was defective in supporting cell-matrix adhesion. We developed an inhibitory monoclonal antibody against QSOX1 that could modulate ECM properties and undermine cell migration.",
author = "Tal Ilani and Assaf Alon and Iris Grossman and Ben Horowitz and Elena Kartvelishvily and Cohen, \{Sidney R.\} and Deborah Fass",
note = "Israel Science Foundation; Israel Cancer Association; Abisch-Frenkel Foundation; Kimmelman Center for Macromolecular Assemblies; European Research Council under the European Union [310649]Y. Yarden provided valued advice and guidance throughout this project. We also thank V. Rotter, S. Feigelson, R. Alon, G. Shakhar, N. Friedman, M. Oren, and B. Geiger for reagents and advice. O. Leitner, Z. Landau, and H. Hamawi from the Weizmann Institute Antibody Unit performed technical aspects of antibody production. T. Mehlman provided mass spectrometry service. K. Shakhar provided statistics support. This work was supported by the Israel Science Foundation, Israel Cancer Association, Abisch-Frenkel Foundation, Kimmelman Center for Macromolecular Assemblies, and European Research Council under the European Union's Seventh Framework Programme (ERC grant agreement 310649). T. I., A. A., I. G., and D. F. have applied for a patent concerning the QSOX1 inhibitory antibody and its use in modulating assembly of laminin in the basement membrane.",
year = "2013",
month = jan,
day = "1",
doi = "10.1126/science.1238279",
language = "الإنجليزيّة",
volume = "341",
pages = "74--76",
journal = "Science",
issn = "0036-8075",
publisher = "American Association for the Advancement of Science",
number = "6141",
}