TY - JOUR
T1 - A role for APETALA1/FRUITFULL transcription factors in tomato leaf development
AU - Burko, Yogev
AU - Shleizer-Burko, Sharona
AU - Yanai, Osnat
AU - Shwartz, Ido
AU - Zelnik, Iris Daphne
AU - Jacob-Hirsch, Jasmine
AU - Kela, Itai
AU - Eshed-Williams, Leor
AU - Oria, Naomi
N1 - Israel Science Foundation [60/10]; U.S.-Israel Binational Agricultural Research and Development Fund [IS 04140-08C, IS-4531-12C]; German-Israel Project Cooperation Foundation [OR309/1-1, FE552/12-1]We thank Eilon Shani (University of California, San Diego) and Moran Farhi (Hebrew University) for help with the yeast one-hybrid assay, Yuval Eshed (Weizmann Institute) for plant material, Tsafi Danieli (Protein Expression Facility Wolfson Centre, Hebrew University) for advice and vectors, and Gideon Rechavi (Chaim Sheba Medical Center) for help with the microarray. We also thank Yuval Eshed, Alon Samach (Hebrew University), David Weiss (Hebrew University), and members of our lab for helpful discussions and Smadar Harpaz-Saad (Hebrew University) and Eilon Shani (University of California, San Diego) for critical reading of the article. This work was supported by grants from the Israel Science Foundation (60/10), the U.S.-Israel Binational Agricultural Research and Development Fund (IS 04140-08C and IS-4531-12C), and German-Israel Project Cooperation Foundation (OR309/1-1; FE552/12-1).
PY - 2013/6
Y1 - 2013/6
N2 - Flexible maturation rates underlie part of the diversity of leaf shape, and tomato (Solanum lycopersicum) leaves are compound due to prolonged organogenic activity of the leaf margin. The CINCINNATA -TEOSINTE BRANCHED1, CYCLOIDEA, PCF (CIN-TCP) transcription factor LANCEOLATE (LA) restricts this organogenic activity and promotes maturation. Here, we show that tomato APETALA1/FRUITFULL (AP1/FUL) MADS box genes are involved in tomato leaf development and are repressed by LA. AP1/FUL expression is correlated negatively with LA activity and positively with the organogenic activity of the leaf margin. LA binds to the promoters of the AP1/FUL genes MBP20 and TM4. Overexpression of MBP20 suppressed the simple-leaf phenotype resulting from upregulation of LA activity or from downregulation of class I knotted like homeobox (KNOXI) activity. Overexpression of a dominant-negative form of MBP20 led to leaf simplification and partly suppressed the increased leaf complexity of plants with reduced LA activity or increased KNOXI activity. Tomato plants overexpressing miR319, a negative regulator of several CIN-TCP genes including LA, flower with fewer leaves via an SFTdependent pathway, suggesting that miR319-sensitive CIN-TCPs delay flowering in tomato. These results identify a role for AP1/FUL genes in vegetative development and show that leaf and plant maturation are regulated via partially independent mechanisms.
AB - Flexible maturation rates underlie part of the diversity of leaf shape, and tomato (Solanum lycopersicum) leaves are compound due to prolonged organogenic activity of the leaf margin. The CINCINNATA -TEOSINTE BRANCHED1, CYCLOIDEA, PCF (CIN-TCP) transcription factor LANCEOLATE (LA) restricts this organogenic activity and promotes maturation. Here, we show that tomato APETALA1/FRUITFULL (AP1/FUL) MADS box genes are involved in tomato leaf development and are repressed by LA. AP1/FUL expression is correlated negatively with LA activity and positively with the organogenic activity of the leaf margin. LA binds to the promoters of the AP1/FUL genes MBP20 and TM4. Overexpression of MBP20 suppressed the simple-leaf phenotype resulting from upregulation of LA activity or from downregulation of class I knotted like homeobox (KNOXI) activity. Overexpression of a dominant-negative form of MBP20 led to leaf simplification and partly suppressed the increased leaf complexity of plants with reduced LA activity or increased KNOXI activity. Tomato plants overexpressing miR319, a negative regulator of several CIN-TCP genes including LA, flower with fewer leaves via an SFTdependent pathway, suggesting that miR319-sensitive CIN-TCPs delay flowering in tomato. These results identify a role for AP1/FUL genes in vegetative development and show that leaf and plant maturation are regulated via partially independent mechanisms.
UR - http://www.scopus.com/inward/record.url?scp=84880946543&partnerID=8YFLogxK
U2 - 10.1105/tpc.113.113035
DO - 10.1105/tpc.113.113035
M3 - مقالة
C2 - 23771895
SN - 1040-4651
VL - 25
SP - 2070
EP - 2083
JO - Plant Cell
JF - Plant Cell
IS - 6
ER -