A protocol for collection and infusion of cerebrospinal fluid in mice

Achint Kaur; Steven Shuken; Andrew Chris Yang; Tal Iram

doi:10.1016/j.xpro.2022.102015

A protocol for collection and infusion of cerebrospinal fluid in mice

Achint Kaur, Steven Shuken, Andrew Chris Yang, Tal Iram

Research output: Contribution to journal › Article › peer-review

Abstract

Here, we provide a step-by-step protocol for the collection and intracerebroventricular infusion of cerebrospinal fluid (CSF) in mice. We describe steps to withdraw CSF quickly and abundantly while avoiding blood contamination. Using the Lynch coil technique, we gain functional insights into the collected CSF by slowly infusing minimal amounts of CSF directly to the lateral ventricles of aged mice. This protocol is versatile and can be used to infuse drugs, antibodies, or scarce biological compounds.

Original language	English
Article number	102015
Number of pages	20
Journal	STAR Protocols
Volume	4
Issue number	1
Early online date	12 Jan 2023
DOIs	https://doi.org/10.1016/j.xpro.2022.102015
State	Published - 17 Mar 2023
Externally published	Yes

All Science Journal Classification (ASJC) codes

General Neuroscience
General Biochemistry,Genetics and Molecular Biology
General Immunology and Microbiology

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10.1016/j.xpro.2022.102015License: CC BY-NC-ND

Cite this

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A protocol for collection and infusion of cerebrospinal fluid in mice

Abstract

All Science Journal Classification (ASJC) codes

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