A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast

Shiran Gabay-Maskit; Luis Daniel Cruz-Zaragoza; Nadav Shai; Miriam Eisenstein; Chen Bibi; Nir Cohen; Tobias Hansen; Eden Yifrach; Nofar Harpaz; Ruth Belostotsky; Wolfgang Schliebs; Maya Schuldiner; Ralf Erdmann; Einat Zalckvar

doi:10.1242/jcs.244376

A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast

Shiran Gabay-Maskit, Luis Daniel Cruz-Zaragoza, Nadav Shai, Miriam Eisenstein, Chen Bibi, Nir Cohen, Tobias Hansen, Eden Yifrach, Nofar Harpaz, Ruth Belostotsky, Wolfgang Schliebs, Maya Schuldiner, Ralf Erdmann, Einat Zalckvar

Weizmann Institute of Science

Research output: Contribution to journal › Article › peer-review

Abstract

Eukaryotic cells have evolved organelles that allow the compartmentalization and regulation of metabolic processes. Knowledge of molecular mechanisms that allow temporal and spatial organization of enzymes within organelles is therefore crucial for understanding eukaryotic metabolism. Here, we show that the yeast malate dehydrogenase 2 (Mdh2) is dually localized to the cytosol and to peroxisomes and is targeted to peroxisomes via association with Mdh3 and a Pex5-dependent piggybacking mechanism. This dual localization of Mdh2 contributes to our understanding of the glyoxylate cycle and provides a new perspective on compartmentalization of cellular metabolism, which is critical for the perception of metabolic disorders and aging.

Original language	English
Article number	jcs244376
Number of pages	10
Journal	Journal of Cell Science
Volume	133
Issue number	24
DOIs	https://doi.org/10.1242/jcs.244376
State	Published - 17 Dec 2020

Keywords

Glyoxylate cycle
Malate dehydrogenase
Peroxisomes
Pex5
Piggybacking
Protein targeting

All Science Journal Classification (ASJC) codes

Cell Biology

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10.1242/jcs.244376License: CC BY

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Gabay-Maskit, S., Cruz-Zaragoza, L. D., Shai, N., Eisenstein, M., Bibi, C., Cohen, N., Hansen, T., Yifrach, E., Harpaz, N., Belostotsky, R., Schliebs, W., Schuldiner, M., Erdmann, R., & Zalckvar, E. (2020). A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast. Journal of Cell Science, 133(24), Article jcs244376. https://doi.org/10.1242/jcs.244376

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title = "A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast",

abstract = "Eukaryotic cells have evolved organelles that allow the compartmentalization and regulation of metabolic processes. Knowledge of molecular mechanisms that allow temporal and spatial organization of enzymes within organelles is therefore crucial for understanding eukaryotic metabolism. Here, we show that the yeast malate dehydrogenase 2 (Mdh2) is dually localized to the cytosol and to peroxisomes and is targeted to peroxisomes via association with Mdh3 and a Pex5-dependent piggybacking mechanism. This dual localization of Mdh2 contributes to our understanding of the glyoxylate cycle and provides a new perspective on compartmentalization of cellular metabolism, which is critical for the perception of metabolic disorders and aging.",

keywords = "Glyoxylate cycle, Malate dehydrogenase, Peroxisomes, Pex5, Piggybacking, Protein targeting",

author = "Shiran Gabay-Maskit and Cruz-Zaragoza, \{Luis Daniel\} and Nadav Shai and Miriam Eisenstein and Chen Bibi and Nir Cohen and Tobias Hansen and Eden Yifrach and Nofar Harpaz and Ruth Belostotsky and Wolfgang Schliebs and Maya Schuldiner and Ralf Erdmann and Einat Zalckvar",

note = "Publisher Copyright: {\textcopyright} 2020. Published by The Company of Biologists Ltd",

year = "2020",

month = dec,

day = "17",

doi = "10.1242/jcs.244376",

language = "الإنجليزيّة",

volume = "133",

journal = "Journal of Cell Science",

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publisher = "Company of Biologists Ltd",

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Gabay-Maskit, S, Cruz-Zaragoza, LD, Shai, N, Eisenstein, M, Bibi, C, Cohen, N, Hansen, T, Yifrach, E, Harpaz, N, Belostotsky, R, Schliebs, W, Schuldiner, M, Erdmann, R & Zalckvar, E 2020, 'A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast', Journal of Cell Science, vol. 133, no. 24, jcs244376. https://doi.org/10.1242/jcs.244376

TY - JOUR

T1 - A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast

AU - Gabay-Maskit, Shiran

AU - Cruz-Zaragoza, Luis Daniel

AU - Shai, Nadav

AU - Eisenstein, Miriam

AU - Bibi, Chen

AU - Cohen, Nir

AU - Hansen, Tobias

AU - Yifrach, Eden

AU - Harpaz, Nofar

AU - Belostotsky, Ruth

AU - Schliebs, Wolfgang

AU - Schuldiner, Maya

AU - Erdmann, Ralf

AU - Zalckvar, Einat

PY - 2020/12/17

Y1 - 2020/12/17

N2 - Eukaryotic cells have evolved organelles that allow the compartmentalization and regulation of metabolic processes. Knowledge of molecular mechanisms that allow temporal and spatial organization of enzymes within organelles is therefore crucial for understanding eukaryotic metabolism. Here, we show that the yeast malate dehydrogenase 2 (Mdh2) is dually localized to the cytosol and to peroxisomes and is targeted to peroxisomes via association with Mdh3 and a Pex5-dependent piggybacking mechanism. This dual localization of Mdh2 contributes to our understanding of the glyoxylate cycle and provides a new perspective on compartmentalization of cellular metabolism, which is critical for the perception of metabolic disorders and aging.

AB - Eukaryotic cells have evolved organelles that allow the compartmentalization and regulation of metabolic processes. Knowledge of molecular mechanisms that allow temporal and spatial organization of enzymes within organelles is therefore crucial for understanding eukaryotic metabolism. Here, we show that the yeast malate dehydrogenase 2 (Mdh2) is dually localized to the cytosol and to peroxisomes and is targeted to peroxisomes via association with Mdh3 and a Pex5-dependent piggybacking mechanism. This dual localization of Mdh2 contributes to our understanding of the glyoxylate cycle and provides a new perspective on compartmentalization of cellular metabolism, which is critical for the perception of metabolic disorders and aging.

KW - Glyoxylate cycle

KW - Malate dehydrogenase

KW - Peroxisomes

KW - Pex5

KW - Piggybacking

KW - Protein targeting

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U2 - 10.1242/jcs.244376

DO - 10.1242/jcs.244376

M3 - مقالة

C2 - 33177075

SN - 0021-9533

VL - 133

JO - Journal of Cell Science

JF - Journal of Cell Science

IS - 24

M1 - jcs244376

ER -

A piggybacking mechanism enables peroxisomal localization of the glyoxylate cycle enzyme Mdh2 in yeast

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