@article{795821be16d04a66b0450dd6586081a0,
title = "A new era for functional labeling of neurons: activity-dependent promoters have come of age",
abstract = "Genetic labeling of neurons with a specific response feature is an emerging technology for precise dissection of brain circuits that are functionally heterogeneous at the single-cell level. While immediate early gene mapping has been widely used for decades to identify brain regions which are activated by external stimuli, recent characterization of the promoter and enhancer elements responsible for neuronal activity-dependent transcription have opened new avenues for live imaging of active neurons. Indeed, these advancements provided the basis for a growing repertoire of novel experiments to address the role of active neuronal networks in cognitive behaviors. In this review, we summarize the current literature on the usage and development of activity-dependent promoters and discuss the future directions of this expanding new field.",
author = "Takashi Kawashima and Hiroyuki Okuno and Haruhiko Bito",
note = "We thank Dr. Paul Worley (Johns Hopkins University), Dr. Kenichi Ohki (Kyushu University), Drs. Masanobu Kano and Kazuo Kitamura (University of Tokyo), and Dr. Itaru Imayoshi (Kyoto University) for continuous collaboration and encouragement. We also thank Drs. Sayaka Takemoto-Kimura, Mio Nonaka, Nan Yagishita-Kyo, Satoshi Kamijo, Aki Adachi-Morishima, and all the members of the Bito laboratory for discussion and support of the research. We apologize to many authors whose work could not be discussed in this article because of space limitations. This work was supported in part by grants-in-aid from the Japanese Ministry of Education, Culture, Sports, Science, and Technology (MEXT) and Japan Society for the Promotion of Science (JSPS; to Hiroyuki Okuno and Haruhiko Bito), grants from CREST–Japan Science and Technology Agency (JST; to Haruhiko Bito), the Strategic International Research Cooperative Program Japan–Mexico (SICPME-JST, to Haruhiko Bito), and the Mitsubishi, Takeda and Uehara Foundation (to Haruhiko Bito). Takashi Kawashima was supported by JSPS fellowships.",
year = "2014",
month = apr,
day = "23",
doi = "https://doi.org/10.3389/fncir.2014.00037",
language = "الإنجليزيّة",
volume = "8",
journal = "Frontiers in Neural Circuits",
issn = "1662-5110",
publisher = "Frontiers Media SA",
number = "APR",
}